The Effect Of G31P On Human Keloid Fibroblasts Proliferation And MMP-2 Expression

Background:Cutaneous scarring is almost an inevitable end of human wound healing,trying closely to both physical scarring and pathological scar.At present,studies mainly focus on hyperplastic scar and keloid,of which the formation and pathological mechanism is complicated,especially the keloid.With tumor-like invasive characteristics,keloid keeps proliferating locally in the body without restraint and infiltrating normal tissue of dermis,leading to pain and itch ect.That will not only ugly,but also cause patients’ mental problems.All of these,including the increasing morbidity and nearly 100% recurrence rate,make it more meaningful to find a perfect solution.Until now,Chemokine and its Receptor has been discovered and named by more than 50 kinds.It becomes a new research hotspot for researchers throughout the world because of their promient position in the form of keloid and potential value to treat pathological scars.CXCR1 / CXCR2 antagonist-G31 P is CXCL8 as the breakthrough point,the use of site directed mutagenesis technology synthetic CXCL8 analogue-CXCL8(3-72)K11R/G31P(G31P),can with CXCL8 receptor CXCR1 and CXCR2 high affinity,and no biological activities,thus simultaneously blocking and CXCR1 and all the chemokine receptor CXCR2 joint,to block the chemotactic factor to promote the process of scarring,providing a new strategy for clinical treatment.Studies show that chemokines and their receptors closely relationship with cancer,such breast cancer,bladder cancer,prostatic cancer,melanoma and so on.In this sense,it is a new way for treatment strategies by exploring its function in the keloid.Objective: To explorethe effect of G31 p for Keloid fibroblasts on proliferation inhibition and the expression of MMP-2,then finding a new treatment plan from the effect of the inhibition of cell proliferation and migration.Methods: 1,Using the tissue block method to train the original generation of keloid fibroblasts,and collecting cells in vitro to observe culture status of cell growth,cell morphology and the ultrastructure of KFBs by the transmission electronmicroscope;2,KFBs are treated with different concentration(0,1,10,100 ng/ml)of G31 p in 24 h,48 h,72 h,then through CCK-8 measuring the cells proliferatin inhibition,measuring inhibition rate,the relationship between drug concentration and time;3,ELISA kit to measure the content of MMP-2 in different concentration G31 p afte24 h,to analyse the effect of G31 p on MMP-2 closely related to cell migration;Results:Selection of 9 cases patients with chest,upper arm,the earlobe department for keloid tissue,successfully cultivate keloid fibroblasts in vitro used in experiments.1,The application of different concentration(1 ng/ml-100 ng/ml)G31P cuture keloid fibroblasts in vitro after 24 h,48 h,72 h,in different time,compared with control group,with increasing concentration G31 P cell inhibition enhance,significant difference was found in 100 ng/ml group than the control group(p < 0.05),and the inhibitory rate decreased with the extension of time;2,Different concentration of G31 P determination of content of MMP-2 contant reduce by effected different concentration G31 P after 24 h,the control group compared with the experimental group,10 ng/ml group significant difference(p < 0.05),,100 ng/ml groupdifference is very apparent,showed G31 P also can inhibit keloid fibroblasts invade migration.Conclusion: 1、G31P inhibit the proliferation of KFBs with a dose-response relationship;2、G31P can reduce the expression of MMP-2 to interpose in the migration of KFBs.