Mechanism Of Poxue Huayu And Huoxue Huatan Kaiqiao Decoction Begin To Understand Law Of VD Rat Hippocampus Of Caspase-3 Expression Effect

Objective:To use modern immunohistochemical technique to explore the method of filling filling pulp,invigorate the circulation of phlegm and begin to understand the effectiveness of the vascular dementia rats and the mechanism of action.Method:1.Choose the SPF Wistar rats,half male and female,weight 220 + 20 g,a total of 60,randomly divided into: control group,model group,positive medicine contrast many fill out fill pith,invigorate the circulation of phlegm begin to understand group,phlegm begin to understand new green particles in high dose group,middle dose group,low dose group,a total of six groups,each group of ten only.Adopt double vascular obstruction(2VO)improvement method to copy the vascular dementia rats model.2.The platform test: using platform experiments to detect vascular dementia rats behavior change.Inspection method of filling filling pulp,invigorate the circulation of phlegm and begin to understand the influence of the vascular dementia rats learning and memory ability.3.Use of immunohistochemical method to detect vascular dementia rat hippocampal CA1 area of caspase 3 protein expression.Result:1.Platform experiment results show that filling filling pulp,invigorate the circulation method of eliminating phlegm to begin to understand the high dosage group and positive control drug aricept group can reduce the number of VD model rats platform experimental error(P < 0.05),to extend the platform test incubation period(P < 0.05).Positive control group and high dose of Chinese traditional medicine group there was no significant difference(P > 0.05).2.High dose of Chinese traditional medicine group of brain vascular dementia rat hippocampal CA1 area of caspase-3 expression compared with model group,less statistically significant(P < 0.05).Positive drug donnelly,pp qi group VD rat hippocampus CA1 area expression of caspase-3 less than model group,with statistical significance(P < 0.05).Highdose of Chinese traditional medicine group compared with positive drug donnelly,pp qi group,no statistical difference.(P > 0.05)Conclusion:Filling pulp,huoxue phlegm begin to understand law formula can obviously improve the vascular dementia rats learning and memory ability,can inhibit the expression of the hippocampus caspase 3,thus reducing the vascular dementia rats cell apoptosis.