| Caused by cerebral ischemia reperfusion of cerebral infarction has a high morbidity, is the leading cause of acquired disability, and the treatment method of acute cerebral infarction is relatively limited. After previous studies found, thrombolysis therapy has good effect for the treatment of acute cerebral infarction by use recombinant tissue Plasminogen Activator(rtPA), has been approved by the food and drug administration(fda) in clinical use. With the promotion and use of thrombolytic therapy, its shortcomings are gradually revealed. By the various aspects of limitation just as the time window, surgery patients, age, physical condition and so on, can't make every patient can timely accept the thrombolysis therapy. And because of the limitation of medical equipment, conditions, most of the local grassroots hospitals cannot smoothly carry out artery thrombolysis, artery should, that makes a large part of the patient can not get treatment timely and makes illness delay, bring huge inconvenience to family and society. Nowadays, our country has entered the aging society, the incidence of ischemic cerebral infarction is also on the rise, in order to reduce the patient pain, make the social stability and family harmony, clinical need to find a way to cure acute cerebral infarction. This experiment through the study of the ischemic preconditioning of SD male rats, observe the glial fibers acidic protein?nerve growth factor?brain derived neurotrophic factor and Nestin expression level and find the link between four factors and glial cells in all time points after ischemia in cerebral infarction area, provide theoretical basis for further clinical research.The SD male rats were randomly divided into sham-operated group, ischemia and reperfusion group, ischemic preconditioning group. The ischemic preconditioning group using line plug method make 10 min of ischemia in rats, after three days make the middle cerebral artery occlusion model. The ischemia reperfusion group three days ago only to expose the neck vessels, then make the rat MCAO model again. The sham-operated group only to expose the neck artery. Every groups in ischemia 2h and 24 h do the neural function defect scale. To compare the change of the infarct volume by 2,3,5- Triphenyltetrazolium Chloride staining method in 24 h,48h,72 h,7d,14 d after ischemia-reperfusion. By hematoxylin eosin staining to observe morphological changes of tissue cells, by immunohistochemical method to test the positive cells expression changes of GFAP, NGF, BDNF, Nestin in infarction area.Compared with sham group, neurological symptom score of ischemic preconditioning group and ischemia and reperfusion group were decreased. Compared with ischemia and reperfusion group, ischemic preconditioning group in postoperative nerve function defect score significantly reduced, and the infarction volume reduction. The ischemic preconditioning group of GFAP NGF Nestin and BDNF were higher than the ischemia and reperfusion group( P<0.05), that have significant difference.To sum up, ischemic pretreatment can effectively protect the ischemia-reperfusion injury, probably by promoting positive cells expression of GFAP, NGF, BDNF and Nestin, and promote the proliferation and differentiation of neural stem cells, to further promote the nerve regeneration. |
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