IL-35 And Treg In The Peripheral Blood And Tissue In Patients With Hbv-related Primary Hepatic Carcinoma

HCC (hepatocellular carcinoma) is one of the most com mon malignant tumors.In china, the chronic hepatitis B vir us infection is the leading cause of liver cancer.Repeated necrosis and regeneration in hepatic cell can be caused by chronic inflammation, causing the regenerative nodules of hepatocytes to liver cirrhosis even liver cancer. In the pr ocess, the different changes in the immune system, about cellular immunity and humoral immunity.The cellular immun ity, especially T lymphocytes play an important role. CD4~+ CD25~+FOXP3~+ regulatory T cells(CD4~+CD25~+FOXP3Treg, Tr eg) is CD4~+ T cell subgroup, specificitly expression of tran scription factor, FOXP3, can secrete IL-10, TGF-beta(TGF-β) and IL-35 cytokines, supress antitumor immune function, play an important role in the tumor immune escape. IL-3 5 belongs to the family of IL-12, the current study con firms that IL-35 is an important inhibitory cytokine,it has been detected in many tumor patients and tumor tissue, b ut the role of IL-35 in the development of hepatitis B rel ated liver cancer have not been reported. Therefore, this p aper aims to explore Treg and IL-35 in the background w ith HBV infection of the role of liver cancer occurrence a nd development process, and its possible mechanism.In this study, we collect 54 cases who had surgical state with i ver cancer in the people’s liberation army 309 hospital ran ge from 2015.04 to 2015.12. The tissue adjacent to carcin oma specimens and the carcinoma were detect Treg, p35 and Ebi3 expression. Finally,we through the enzyme-linked immunosorbent assay (enzymelinkedimmunosorbentassay, ELISA) to detcte the serum levels of IL-35 in hepatocellu lar carcinoma patients and 15 cases as control group. Res ults show that:1, IL-35 in carcinoma patients peripheral blood protein expression levels higher than normal people, and the difference was statistically significant (p< 0.05).2, In hepatocellular carcinoma patients’peripheral blood, the level of IL-35 is closely related to tumor size and TNM staging.3, Immunohistochemical results showed that the liver cancer cells can express p35 and Ebi3.A tentative inference on this result is liver cancer cells may secrete IL-35.4,Confirmed that Treg in hepatocellular carcinoma patients peripheral blood and tumor tissue levels higher than normal, and the level of IL-35 is closely related to Treg.5, WB and immunohistochemical results suggest p35 and EBi3 expression in liver cancer tissue is higher than normal liver tissue or adjacent to carcinoma, that was found to be inconsistent with the RT-PCR results,the change of p35 and Ebi3 were be considered regulating gene transcription, causing the differennce of protein expression and gene levelThrough this study the following conclusions:1, A large number of Treg cells is detected in liver cancer tissue and peripheral blood, Treg may participate in the hepatitis b related to liver cancer development.2, the level of p35-mRNA and Ebi3-mRNA are diferent with WB results, on the other hand, may p35 and EBI3 in the process of transcription occurs at some regulation.3,IL-35 in hepatocellular carcinoma patients peripheral blood is closely related to Treg.4,The level of IL-35 is closely related to tumor size and TNM staging,that is show IL-35 may participate in the occurrence of liver cancer development. Through a series of experiments,we can preliminary evidence that Treg and related cytokines IL-35 in hepatitis B correlation play an important role in the development of liver cancer, providing theoretical basis for targeting therapy of liver cancer patients.