The Correlation Of AKR1B10 Expression In Liver Cancer Tissue And Hepatitis B Virus Infection

ObjectiveTo explore correlations between the expression levels of Aldo-keto reductase family 1 member B10(AKR1B10) in liver cancer tissue and hepatitis B virus(HBV) immune markers and HBV-DNA copies.Methods93 cases of hospitalized patients with clinical pathology diagnosis of liver cancer were investigated, and their serum samples and liver cancer tissues were collected. The expression levels of AKR1B10 protein in the tissue samples were detected by immunohistochemistry staining method. HBV immune markers(HBV-M) and the HBV-DNA amount were examined respectively by time-resolved immunofluorescence analysis method and by PCR fluorescence probe method, and serum AFP amount was detected by direct chemiluminescence method. HBV-DNA quantitative results were described as a base-10 logarithm of LG(HBV-DNA). The expression levels of AKR1B10 were analyzed between HBV-positive and HBV-negative groups. The correlations were analyzed between the expression levels of AKR1B10 in liver cancer tissues and serum HBV-DNA copies and serum AFP levels.Results1. The expression rate of AKR1B10 protein in liver cancer tissues was 67.7%(63/93). There were not significant different between AKR1B10 expression levels and clinic pathological parameters of patients such as age, gender, tumor diameter, satellite lesions, differentiation, liver is membrane invasion and cirrhosis(p>0.05).2. The expression levels of AKR1B10 protein were significant different between AFP-positive and AFP-negative groups(?2=3.961, p<0.05).3. The expression levels of AKR1B10 protein in liver cancer tissues were not different between HBV-M-positive and HBV-M-negative groups(p>0.05). AKR1B10 expression levels were significant difference between HBV-positive and HBV-negative groups(?2=4.072, p<0.05). The expression level of AKR1B10 protein in liver cancer tissues was positively correlated with the amount of serum HBV-DNA(r=0.332, p<0.01).Conclusion1. There was the correlation between AKR1B10 expression in liver cancer tissue and hepatitis B virus infection.2. There was a positive correlation between the expression level of AKR1B10 protein in liver cancer tissue and the degree of activity of HBV replication.