Research On The Impact Of Borneol On Blood Brain Barrier And P–glycoprotein

Objective: to study the effect of different doses of borneol on phenytoin sodium concentration in serum and brain tissue;to study the influence of different doses of borneol on the amount of P- glycoprotein expressed in the small intestine and brain tissue;to explore whether borneol can promote the drugs into the blood brain barrier and enhance the therapeutic effect.Method: 1. To feed rats on borneol in10 times, five times and one time clinical equivalent dose, and get the determination of concentrations of phenytoin sodium in serum and brain tissue of different groups by high performance liquid chromatography. 2. To measure concentrations of P- glycoprotein in small intestine and brain tissue of borneol in different drug delivery dosage groups by enzyme-linked immunoassay. 3. To detect positive expression of P-glycoprotein in the small intestine of borneol in different dosage dose group by immunohistochemical method.Result: 1. Compare phenytoin sodium concentration in serum and brain tissue of 10 times the dose of borneol to of five times the dose group,the P values are: 0.2269?0.2537. compare phenytoin sodium concentration in serum and brain tissue of the five times the dose of borneol to of one time dose group,the P values are: 0.0009?0.0175. 2.compare P- glycoprotein concentration in serum and brain tissue of 10 times the dose of borneol to five times the dose group,the P values are: 0.1819?0.0000.3. Borneol different dose groups of small intestine P- glycoprotein positive expression quantity is higher than the phenytoin sodium group.Conclusion: borneol can promote the phenytoin sodium through the blood brain barrier, increase the phenytoin sodium concentration in serum, brain and Borneol can inhibit the expression of P- glycoprotein in small intestine, brain tissue, reduce the amount of positive expression, promote the drugs into the blood and into the brain, to ensure effective treatment.