Xiaoqinglongtang On The Cold Asthma Mice Of TSLP Inflammation Factor Regulation Function

Objective:Successful cold asthma mice model is set up; Xiaoqin-glongtang on TSLP at information transmission loop (TNF-α-NF-KB-TSLP-Th2-TNF-α), the influence of the found targets of Xiaoqinglon-gtang; To test whether Xiaoqinglongtang and the mechanism of action of glucocorticoid to TSLP at the same, combined with the use of mo-lecular biology, pathology, the Xiaoqinglongtang curative effect mecha-nism of cold sex asthma model in mice, whether done by the control factor of arrives, and looking for targets, provide theoretical basis fo-r Xiaoqinglongtang treatment of asthma.Methods:Female white variant laboratory mice 50, SPF, weight 20 to 22g, r at ages 6 to 8 weeks. Randomly divided into 5 groups:b-lank group (group A), model control group (group B), Xiaoqinglongta-ng group (group C), dexamethason e group (group D), Xiaoqinglongtang + dexamethasone group (group E),10 in ea ch group. Cold asthma model:with ovalbumin sensitization and aluminum hydroxi de powder s-uspension solution in mice, and to stimulate mice ovalbumin solution; After the success of the induced asthma mice performance:start acti-vity is freq uent, restlessness; Then bristling, less prone, respiration, depression; Moreover ch est ups and downs, and neck, mainland incontinence, etc.; According to the above method makes mice after sensitization, at the same time of using OVA atomized trigger asthma, put mice at the temperature of -4-4℃ environment, to give air c. onditioning cold wind blowing. Mice on the basis of the above performance appe ars evil wind chill, arch and standing hair, sneezing, runny nose and other cold s ymptoms, for building successful. Drug intervention experiment:Xiaoqinglongtang group:since inspired day 1, day serving Xiaoqinglongtang the original side, und ertake convert by normal 9.1 times the human dose,1 x per day, one of 0.9g/100 g, continuous 7d; Dexamethasone group:since inspired day 1, by intraperitoneal i njection of dexamethasone 3mg/kg/d, continuous 7d;Xiaoqinglongtang+dexamethas one group:since inspired day 1, day serving Xiaoqinglongtang the original side, undertake convert by normal 9.1 times the human dose,1 x per day, one of 0.9g/ 100g, at the same time by intraperitoneal injection of dexamethasone 3mg/kg/d,7 d in a row. At the end of the time within 12 hours after the following:pick eye ball blood, serum, centrifugal to return to return the lung tissue dissection mice. Groups of mice serum IL-1p and TNF-a content, serum and lung tissue in mice, the content of the NF-κB and TSLP at in and do the lung tissue pathology exami nation, to observe the lung tissue of mice inflammatory cells infiltration and path ological changes of the airway.Results:Compared with the blank group, model control group, IL-1β, TNF-α,NF-κB,TSLP at levels were elevated, with statistical significance(p>0.05);Compa red with model group, the Xiaoqinglongtang group, dexamethasone group, the Xia oqinglongtang+dexamethasone group can reduce the IL-1β, TNF-α, NF-κB, TSLP at levels, with statistical significance (p<0.05); Compared with Xiaoqinglongtang group, dexamethasone group IL-1β, TNF-α, NF-κB, TSLP at the level of change is no difference, no statistical significance(p>0.05), Xiaoqinglongtang+dexamethaso ne group of IL-1β, TNF-α, NF-κB, TSLP at levels were lower, there is statistical significance, (p<0.05); Compared with dexamethasone group, Xiaoqinglongtang+d examethasone group of IL-1β, TNF-α, NF-κB, TSLP at the level of both decrease d, with statistical significance (p<0.05).Conclusion:1, Xiaoqinglongtang can cut the level of TSLP,TNF-α, IL-1β,NF-κB of cold asthma mice.2, Xiaoqinglongtang by inhibiting ring information guide pathway for the treatment of asthma, and treatment with glucocorticoid. Xiaoqing longtang in the treatment of asthma effects by inhibiting TSLP ring information g uide pathway (TNF-α-NF-κB-TSLP-Th2-TNF-α), and treatment with glucocorticoi d.