Preparation And Evaluation Of Polyphenols-Loaded Nano/Micro Carriers

Both tea polyphenols and resveratrol are plant polyphenols as well as strong anti-oxidant. Tea polyphenols derived from tea are water solubility active matter. They have anticancer, anti-aging, anti-inflammatory effect. Besides, they could also protect angiocarpy and inhibit melanin. However several physicochemical properties, such as instability, easy oxidation degradation, sensitivity to light and alkaline solution, etc., limit its application. Resveratrol is fat-soluble active matter which is derived from plant, such as grape, polygonum cuspidatum and so on. It also has anticancer, anti-aging, anti-inflammatory, coronary heart disease preventing and skin whitening properties. However, its physicochemical properties, such as poor water solubility, poor stability, solder oxidation photosensitivity and isomer structure, restrict the application in the field of the food, cosmetic and pharmaceutical. Therefore, in this paper, three kinds of carriers were developed. The first one is the multiple solid particles (MLPs) encapsulated tea polyphenols, which improve the stability and bioavailability of tea polyphenols. The second one is the MLPs which both tea polyphenols and resveratrol were packaged in the inner water phase, enhancing the stability and bioavailability of tea polyphenols and resveratrol and improving the water solubility of resveratrol. The third one is the lipid nanocapsule encapsulated resveratrol, increasing the stability, water-solubility and load of resveratrol.This paper first fabricated tea polyphenols multiple solid particles (TP-MLPs) using the improved two-step emulsification method. The best formula was selected based on the evaluation of emulsification ability and emulsion stability index (ESI). Then the microstructure of carrier and the encapsulation efficiency and retention rate of tea polyphenols were investigated. The experimental results showed that encapsulation efficiency of TP-MLPs we developed is high. In vitro release study displayed a good sustained release of TP-MLPs, and in vitro penetration studies promoted the accumulation of tea polyphenols in the skin.The latent solvent was first screened, then tea polyphenols and resveratrol were mixed using latent solvent and water to form a uniform solution as the inner water phase. Then the tea polyphenols/resveratrol multiple solid particles (TR-MLPs) was developed. The vitro release study and vitro penetration studies of TR-MLPs were investigated later and the encapsulation efficiency was high. The experiment results showed that TR-MLPs was a good carrier which could increase the retention rate of active matter in skin and displayed a good sustained release.The hot high-pressure homogenization method (HPH) was used to produce resveratrol lipid nanocapsule (Res-LN), which had transparent appearance and uniform particle size. The experiment results showed that this carrier had high centrifugal stability and dilution stability. Percutaneous behavior showed that this carrier had a strong affinity to the skin and could promote skin absorption of resveratrol.