Comparison Of The Effects Of Oxycodone Hydrochloride, Morphine Hydrochloride On Immune Function Of Mice Through The Intraperitoneal Injection

Objective The aim of the study was to explore and compare the effects of oxycodone hydrochloride, morphine hydrochloride on the immune function of mice. Methods 30 male ICR mice which were 5 weeks old were randomly divided into three groups:the morphine group (n=10), oxycodone group (n=10) and saline control group (n=10). The morphine group was injected intraperitoneally with morphine hydrochloride according to the dose of 40mg/kg. The oxycodone group was given the oxycodone hydrochloride intraperitoneally according to the dose of 40mg/kg. The saline control group was injected intraperitoneally with normal saline according to the dose of 40ml/kg. These drugs were given for 9 days at the same time everyday.24 hours after the last injection, these mice were weighed and then killed. Under sterile conditions, the spleens and thymuses of these mice were taken out and weighed. After the preparation of single cell suspension of the spleens and thymuses, the lymphocyte subsets of these cell suspensions were measured by flow cytometry.Results There were no significant differences among the spleen index of the three groups(P>0.05). The thymus index of the morphine group was significant lower than that of the saline group(P<0.01), it was also lower than that of the oxycodone group(P<0.05). There was no significant difference between the thymus index of the oxycodone group and the normal saline group(P>0.05). The proportion of CD3+ T cell of the oxycodone group was significantly higher than that of the saline group(P<0.01) and the morphine group(P<0.01). The proportion of CD3+ T cell was also significant different between the saline group and the morphine group(P<0.05). The proportion of CD19+ B cell of the morphine group was significantly lower than that of the saline group(P<0.05) and the oxycodone group (P<0.01). The proportion of CD8+ T cell of the oxycodone group decreased significantly than that of the saline group(P<0.05), while the ratio of CD4+ T cell of the oxycodone group was significantly higher than that of the normal saline group(P<0.01) and the morphine group (P<0.01). The ratio of double positive T cell of the morphine group was significantly higher than that of the normal saline group(P<0.01), it was also higher than that of the oxycodone group(P<0.01). At the same time, the ratio of CD4+/CD8+ of the oxycodone group was significantly higher than that of the normal saline group (P<0.01) and the morphine group (P<0.05).Conclusions Morphine hydrochloride could significantly inhibit the immune function of mice, while Oxycodone hydrochloride had no obvious inhibitory effects on the immune function of mice. Oxycodone hydrochloride could promote the T cells develop to CD4+ T cell, it also increased the proportion of peripheral T cells.