Preparation And Evaluation Of Multi-Vitamin Loaded Nano/Micro Carriers

Vitamin C and vitamin E, which can only be provided exogenously, are both important natural antioxidants for human body. Although many cosmeceutical formulations contain vitamin C and/or vitamin E, very few are actually effective in topical application. First because there is only a low concentration, second because the stability is compromised as soon as the product is opened and exposed to air and light, and third because the form of the molecule (an ester or a mixture of isomers) is not absorbed or metabolized effectively by the skin.However, when a stable formulation delivers a high concentration of the nonesterified, optimal isomer of the antioxidant, vitamins C and E do indeed inhibit the acute ultraviolet (UV) damage of erythema, sunburn, and tanning as well as chronic UV photoaging and skin cancer. Both are highly effective depigmenting agents. Topical vitamin C also increases collagen synthesis in both young and old fibroblasts. Because vitamin C regenerates oxidized vitamin E, the combination in a cosmeceutical formulation is synergistic-particularly in UV protection. Solid lipid microparticle carriers (SLMs), combined with the advantages of double emulsions (W/O/W) and water-in-oil micro emulsions, are developed for transdermal administration. A modified one-step emulsification method was introduced, which made the industrial production of SLMs easier. Variables involved in preparation that may affect the solubility, stability and entrapment efficiency of SLMs were investigated. With vitamin C and vitamin E as model actives, the advantages of SLMs in transdermal administration were studied further. Vitamin C, encapsulated by SLMs, exhibited an enhanced storage stability up to 3 month under 25℃ and a sustained releasing profile over 24 h. Furthermore, the SLMs-encapsulation of vitamin C, E was very helpful in delivering vitamin C and E molecules into skin through stratum corneum. The result showed 5.52- and 7.28-fold (p<0.05) increased absorption of vitamin C and E, compared to the vitamin C and E solution respectively. MTT cell toxicity test proved that SLMs had no toxicity on L929 cell when the dilution ratio was above 100 times.On the basis of previous studies, the effects of preparation methods were also investigated in terms of physical property, stability, superoxide anion scavenging activity, release profile, skin penetration and cell toxicity. The result showed that SLMs prepared by two-step method showed better long-term stability and higher cell toxicity than by on-step method. These studies demonstrate that SLMs may be a promising method for facilitating transdermal penetration of active ingredients with high solubility and low permeability.In addition, Vitamin E and Vitamin A Palmitate loaded snake oil lipid nanocapsules (LNCs) was developed. The particle size, PDI, zeta potential, pH of this system were characterized. Long term stability study showed that the Multi-Vitamin-Snake oil-LNCs were of great stability. Vitamin E (2%) can protect the snake oil form lipid peroxidation effectively. Besides that, Multi-Vitamin-Snake oil-LNCs also had good skin penetration ability and excellent moisturizing efficacy. The nanoscale particle size and components of LNC are highly related to its function.