Role Of Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 In Ox-LDL Induced Apoptotic In Hepatic Sinusoidal Endothelial Cells

Objective:The aim of this study was to investigate the role of lectin-like oxidized low-density lipoprotein receptor-1 in ox-LDL induced apoptotic in hepatic sinusoidal endothelial cells.Methods:Cells were treated with different concentration of ox-LDL(0.10.40.80 ug/ml).3 parallel aperture were set up in each group.After incubation of HSECs for 6?12?24 h,MTT assay were used to analyze the effect of ox-LDL on HSECs viability;Apoptotic morphology changes were studied by Hochest 33258 staining.And apoptosis of HSECs was measured with flow cytometry.Real-time polymerase chain reaction(RT-PCR)and western-blotting were employed to detect the expression of Bax and Bcl-2 in HSECs from the level of mRNA and protein respectively.Results:Compared with contorl groups,the viability of cells were markedly decreased when HUVECs were treated with different concentration ox-LDL(P<0.05),within 24 hours after treatment with different concentration ox-LDL,the cell nuclear condensation and formation of apoptofic bodies were found by morphological observati.Meanwhile,apoptotic rate in ox-LDL treated group was significantly higher than that of control group in the same manner(P<0.05).When treated with different concentration ox-LDL(0.10.40.80 ug/ml)the expression of pro-apoptotic protein Bax was up-regulated(P<0.05).While,the anti-apoptotic protein Bcl-2 was down-regulated in a time-dependent and dose-depended manner in HHSECs(P<0.05).However,polyinosinic cytidylic caid(250ug/ml)restrained apoptotic induced by ox-LDL(P<0.05).Conclusion:Oxidized low-density lipoprotein(ox-LDL)could induce apoptosis in a time-dependent and dose-depended manner in HHSECs,which might be associated with its receptor LOX-1.