| Objective To investigate the effect of chondrocytic biomimetic matrix scaffold materials containing type II collagen(COL II) hydrogel, chondroitin sulphate(CS) and(or) hyaluronic acid(HA) on chondrogenic differentiation of rat bone marrow mesenchymal stem cells(BMSCs) in vitro.Methods BMSCs were cultured in different groups as following: Control,COL II,COL II-CS,COL II-HA,COL II-CS-HA. On day 7, 14, 21 and 28,cell proliferation and differentiation activities were tested using CCK-8 assay. The mRNA and protein expression of ColII, sex determining region Y(SRY)-related high mobility group-box gene 9(SOX9) and aggrecanin were detected using reverse transcription PCR and immunofluorescence technique.Results The result of CCK-8 assay indicated chondrocytic biomimetic matrix scaffold materials based on COL II could be used as good extracellular matrix for BMSCs culture in vitro, CS and HA could increase the proliferation and differentiation activities; All the groups could induce BMSCs to differentiate into chondrocyte; COL II-CS-HA-coated group could up regulate the mRNA and protein expression of COL II, SOX9 and aggrecan most effectively.Conclusion Chondrocytic biomimetic matrix scaffold materials based on COL II could effectively induce rat BMSCs to differentiate into chondrocyte-like cells in vitro, supplementation of the hydrogels with both CS and HA had the maximum efficacy. |
