| Objective: Observe the effects of artemisinin on cardiac inflammation and fibrosis in diabetic cardiomyopathy rats.Methods: 35 male Wistar rats were randomly divided into 2groups: normal group(n=10) and experiment group(n=25)which received a single intraperitoneal injection of streptozotocin at the dose of 65 mg / kg. The rats of normal group received citrate buffer alone(PH 4.2). One week later,fasting plasma glucose levels were measured, The rats whose blood glucose were at least twice higher than 16.7 mmol/L and characterized by polydipsia, polyphagia, polyuria, were considered as diabetic model. All rats were fed for 16 weeks after STZ or citrate buffer injections and had free access to standard rat diet and water. The DCM rats were randomly divided into 2 groups: DCM group, artemisinin treatment group. 21 DCM rat models were established. In week16,Artemisinin was administrated by gavage(75mg / kg) once a day for 4 weeks. The Artemisinin was dissolved in 0.5%sodium carboxymethyl cellulose solution, the rats of normal group and diabetic cardiomyopathy group were treated withsame dose of sodium carboxymethyl cellulose. During the process of treatment, body weight and blood glucose were measured, Cardiac function were measured in week 16 and 20.The myocardial pathology structure were observed by HE staining. The content of collagen were measured by Masson staining. Immunohistochemistry was used to measure the expression of TGF?-1, Collagen ?, Collagen ?, TNF-?,IL-1?, NF-KB in each group.Results:(1) General features of the experimental rats: At the end of the experiment,10 control rats,9 DCM rats,10 ART rats were survived.(2) General condition:Normal rats had white smooth fur and robust body. While the rats of DCM group had a dark yellow fur, suffering from Polydipsia, polyphagia and polyuria,as well as significant weight loss than normal group(P<0.01). Artemisinin treatment group had a yellowish fur, Polydipsia, polyphagia and polyuria reduced and had more weight than DCM group(P<0.01).(3) Plasma Glucose : Normal blood glucose maintained between 3.7 to 7.3. Compared with the normal group, glucose Level of DCM group increased significantly(P<0.01). Glucose Level of The Artemisinin treatment group has no significantly different with normal group(P>0.05).(4) Cardiac function : Compared with DCM group,the LVESD and LVEDD of ART(Artemisinin) treatment group decreased significantly(P<0.01), LVEF and FS increased significantly(P<0.01), E/A increased(P<0.05).(5) HE staining : Myocardial of normal group were wellarranged and distributed.The nuclei were obvious, staineduniform. There were no myocardial hypertrophy, fracture in normal group. The myocardial damage, fracture significantly and can be seen many small necrosis in DCM group. Compared with DCM group, necrosis in Artemisinin treatment group was significantly reduced.(6) MASSON staining : Compared with normal group,the red myocardial fracture, damage obviously,the expression of collagen fibers increased significantly in DCM group(P<0.01). In ART(Artemisinin) group, red cell morphology is substantially complete. The expression of collagen fibers in Artemisinin treatment group had a lower level than in DCM group(P<0.01).(7) immunohistochemistry : TGF?-1,Collagen?,Collagen?,TNF-?,IL-1?, NF-KB are all have a very low expression in myocardial of normal group. Compared with normal group, the myocardial inflammation and fibrosis levels all increased significantly(P<0.01)in DCM group. The Myocardial of Artemisinin treatment group have a lower expression than DCM group in TGF?-1(P<0.01)?Collagen?(P<0.01)?Collagen?(P<0.05)? TNF-?(P<0.01)and NF-KB(P<0.01).But IL-1?has no statistical differences compared with DCM group(P>0.05).Conclusion:(1) Artemisinin can improve General condition and cardiac function of DCM rats.(2) Artemisinin can reduce the expressions of TNF-? and NF-KB, decrease cardiac inflammation of diabetic cardiomyopathy rats.(3)Artemisinin can reduce the TGF?-1, Collagen ?,Collagen ? expression levels and reduce the myocardial fibrosis. |
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