The Preparation Of Forced Swim Stress Induced-Premenstrual Dysphoric Disorder With Liver-Qi Depression Rat Model And The Change Of Neurotransmitters And Their Receptors In Rat Models

Purpose: Rats with Premenstrual dysphoric disorder were prepared and identified in line with the diagnostic criteria for PMDD primary liver qi depressive syndromes property, and by disease syndrome rat model study, culminating in PMDD liver depressive syndrome rat model modeling and evaluation of technical specifications. At the same time, with the model as a carrier, By examining the changes of the brain neurotransmitter content and the receptor gene and protein expression which is closely related to the disease, we can probe its pathogenesis preliminarily.Method: This study with the method of animal behavior experiments and neural biochemical tests which is a combination of method to explore our wants.(1)Using forced swimming test methods, test the fist day of Non-Receptive phases and the Receptive phases. Sequentially test the fist day of Non-Receptive phases, Receptive phases, the fist day of Non-Receptive phases, a total of three times, each time interval of two cycles or 9 days selected Immobility time and other acts parameters having estrous cycle-dependent rats;(2)Surgical removal of the screened out in ovariectomized rats, the disappearance of such acts phenotype;(3) Ovariectomized rats with exogenous sex hormones to induce artificial cycle, the behavior Syndrome phenotype appeared;(4) Treat the "model" of rats induced by artificial cycle above with fluoxetine and ShuYu capsule, to observe the behavior parameters dependence on estrous cycle disappeared, such as the Immobility time. With strict scientific evidence to prove that the disease syndrome rats model belongs to PMDD liver qi depression disease model in rats;(5) After drug intervention,using ELSA kits to test the variation in content of 5-HT, NE, GABA in each group in serum and part of the brain, and using RT-PCR, western-blot method to detect the gene and protein expression of GABAAR4?, to preliminary probed the neural biochemical mechanism.Results:(1)With the method of forced swimming,we get the symptoms of model rats having estrous cycle dependent:Between Receptive phases and Non-Receptive phases, immobility time and immobility number has significant difference(P<0.05). OT% and OE% are also have significant difference in EPM(P<0.05). Model group and control group with significant difference in SPT.(2) Ovaries removed, phenotypic differences disappear between Receptive phases and Non-Receptive phases(P>0.05).(3) After artificial cycle induced by exogenous hormones, the immobility time?immobility number?OT% and OE% of model group rats appeared significant difference(P<0.05,P<0.01). (4) After drug intervention, the phenotypic differences only model group(P<0.05), the differences of two drug groups disappeared(P>0.05).(5) According to the results of ELSA kit, 5-HT content in peripheral blood serum and brain regions, the model group than the control group significantly reduced(P<0.05,P<0.01); Fluoxetine group compared with model group has increased significantly except the hippocampal area(P<0.05); ShuYu capsule group compared with model group significantly increased in the hypothalamus(P<0.05), the rest of the brain and serum showed a rising trend. NE in peripheral blood serum of model group significantly increased than the control group(P<0.05), administration group decreased significantly in the model group(P<0.05); Results in the brain areas, on the other hand, model group than the control group significantly reduced(P<0.05), Fluoxetine group in the hypothalamus and hippocampus in model group significantly increased(P<0.05), ShuYu capsule group compared with model group in the frontal lobe and hippocampus increased significantly(P<0.05). The content of GABA in frontal lobe, hypothalamus, hippocampus,,model group than the control group significantly reduced(P<0.05,P<0.01), And two administration group significantly increased than the model group(P<0.05,P<0.01). According to the results of Western-Blot and RT-PCR, The gene and protein expression of GABAAR4?, model group compared with the control group were significantly increased(P<0.01), Fluoxetine and ShuYu capsules administration group compared with model group significantly reduced(P<0.05,P<0.01).Conclusions:(1) The PMDD liver qi depressive diseases rat model screening forced swimming method,conform to PMDD primary standard:Non-Receptive phases(similar to the previous) "symptoms appear"; Receptive phases(similar to the follicular phase) "symptoms reducing or disappear". And through the complete chain of evidence to confirm the reliability of this method.(2) In The PMDD liver qi depressive, depression and anxiety emotion occurs at the same time, they both have estrus dependencies. The pathogenesis of the disease is closely related with single amine neurotransmitter, such as 5-HT, and the gene and protein expression of GABAAR4?.