Preparation Of Multifunctional ¹⁹F Magnetic Resonance Imaging Nanoprobes And Their Applications In Cancer Diagnosis And Treatment

Because of its non-invasion, endogenously negligible background and high spatial resolution,19F magnetic resonance imaging (19F MRI) has attracted more and more attentions in the field of bio-imaging, disease diagnosis, and so on. Meanwhile, multifunctional nanocomposite probes also showed tremendous potential and desirability in tumor diagnosis and treatment. In this work, we focused on the composition of the organic fluorine ligands with inorganic nanoparticles, in order to fabricate highly fluorine atoms loaded nanocomposites as multifunctional nanoprobes for tumor imaging and diagnosis. Additionally, we have successfully developed a general and facile strategy for the preparation of multifunctional 19F MRI nanoprobes, which paved the way for future developments of new methods and materials in bio-imaging and disease diagnosis.Herein, we have successfully synthesized a multifunctional nanoprobe (Cu7S4@PSI-19F) with ultra-high fluorine payload on the surface of Cu7S4. In vitro investigations demonstrated excellent sensitivity for 19F MRI of these nanoprobes, as well as high efficiency of infra red photothermal conversion. The fluorine contained monomer (3,5-bis(trifluoromethyl)benzaldehyde) was first modified with oleylamine (OAm) to form the hydrophobic chain(19F0Am)-These as-synthesized fluorine ligands were mixed with Cu7S4 nanoparticles and OAm modified polysuccinimide (PSIOAm), and then emulsified and self-assembled via hydrophobic interactions under sonication and alkaline conditions to form the multifunctional nanoprobes. This one-step method made it possible to overcome the hydrophobicity, toxicity,19F MR signal amplification and phase transfer of the fabricated 19F MRI nanoprobes. In vivo assessments of tumor-bearing mice further demonstrated the strong 19F MRI signal in the tumor sites, as well as effective inhibition of the growth of tumor after near-infrared (808 nm) photothermal treatment.In addition, we presented a general and facile strategy to fabricate multifunctional nanoprobes with 19F MRI modality. Perfluorinated silane (1H,1H,2H,2H-perfluorodecyltriethoxysilane, PDTES) with a long hydrophobic chain and oleylamine modified PSI (PSIOAm) were used to co-encapsulate different kinds of inorganic nanoparticles via single particle per micelle under alkaline and sonication conditions. With the further hydrolysis of PDTES and PSI in the basic sulotion, a desirable hydrophilic layer was formed on the surface of the probes, which facilitates the fabrication of biocompatible and stable nanoprobes in water media. These nanoprobes were endowed with 19F MRI modality, meanwhile, the shape, size, and chemical composition, as well as optical and magnetic properties of the nano cores were well retained. This universal method opened up new ways for the multifunction alization of inorganic nanomaterials and their bioapplications.