Research On The Drug Nanocarriers Based On Graphene Oxide And Mesoporous Silica

Recently, nanomaterials has been applied in biomedical fields including drug delivery and treatment, disease test and diagnosis more and more widely. Especially in the anti-cancer drug delivery, the nanomaterials have gained great advance so that they have attracted more and more concentrations in the biomedical domain. This article mainly compared and analyzed the characters mesoporous silica nanoparticles and graphene oxide, which conduct as anti-cancer drug nanocarners.Firstly, prepareing mesoporous silica nanospheres by the modified reverse micelles experiment and researching the effect of their size and morphology on drug encapsulation and release as well as the cell endocytosis. The results confirmed that both of the mesoporous silica nanoparticles possessed excellent encapsulation for anti-cancer drug-hydrochloride doxorubicin (DOX). However the drug release property, dispersity and stability of the different mesoporous silica nanoparticles exist definite difference. As the drug nanocarriers, the mesoporous silica nanosphere which performed more slowly and steady in drug release process and higher drug release amount possessed more excellent dispersity and stability compared with the mesoporous silica nanorods.Next, this article selected graphene oxide, mesoporous silica nanoparticles and graphene silica nanosheets which combined the above two nanomaterials together as the research objects to study and analyze their cell endocytosis, cell toxicity, drug encapsulation and release as well as the morphology stability. The results manifested that the graphene oxide and mesoporous silica nanoparticles were endocytosed by the the endocytosis mediated by clathrin. The graphene silica nanosheets were endocytosed by both the endocytosis mediated by clathrin and pinocytosis. Considered comprehensively, as anti-cancer drug nanocarriers each nanoparticle possessed their own characteristics and advantages.At last, this article studied the drug encapsulation and release, cell endocytosis and cell toxicity of the mesoporous silica nanpsheets conducted as drug nanocarriers. The experimental results showed that mesoporous silica nanosheets have higher drug encapsulation, but relatively low drug release and prompt drug release behavior. During the endocytosis by HeLa cells, this nanomaterial which owned excellent biocompatibility was endocytosed by both the endocytosis mediated by clathrin and pinocytosis. After the entry into HeLa cells, the nanoparticles would experience metabolic process for some time and terminally were all exocytosed out of the cells, nevertheless, the sheet structure lowered the stability of their morphology. Afterwards, the mesoporous silica nanpsheets were modified with the hyaluronic acid(HA),and then the drug encapsulation and release, targeting specificity and cell toxicity of the modified nanoparticles were experimented. After the modification of the HA, the drug encapsulation was lower compared with before, yet the release property has been improved obviously which even represented pH and redox double response characteristics and higher release along with the tardy release process.For the endocytosis, because of the specific combination of hyaluronic acid and glycoprotein CD44 which overexpressed on the cancer cell surface,the mesoporous silica nanosheets modified hyaluronic acid on surface achieved the specificity to target cancer cells and improved the amount of the nanoparticles endocytized by the cells. Take the excellent release property, targeting specificity and biocompatibility into consideration,the mesoporous silica nanosheets were regarded as one ideal nanocarriers for the anti-cancer drug delivery.