| [Objective]Pancreatic cancer serum and bile CA19-9,the expression of PARP-1 related proteins in pancreatic cancer tissue and peri-cancer tissue,and the relationship between PARP-1 Val762Ala SNPs and susceptibility of pancreatic cancer were studied in our research,as well as the clinical diagnosis value of PARP-1 combined with serum and bile CA19-9.[Method]66 Post operated pancreatic cancer patients which were treated with surgery from Jan 2012 to Jun 2013 were recruited in our study.85 patients which were diagnosed with pancreatisis were recruited as the control samples.Serum blood were collected by using venipuncture.5-10mL bile was collected by using endoscopic retrograde cholangio-pancreato graphy(ERCP).Post operated cancer tissue and peri-cancer tissue were collected too.CA19-9 in bile and serum,the relation between SNPs of PARP-1 and the susceptibility of pancreatic cancer,and the level of PARP-1 related proteins in pancreatic cancer tissue and peri-cancer tissue were detected by using automatic electrochemical luminescence analyzer,Mass ARRAYTM Analyzer and immunohistochemistry respectively.[Results]The level of serum and bile CA19-9 in pancreatic cancer group was significantly higher than that in pancreatitis group(P<0.05).The analysis of ROC curve showed that serum and bile CA19-9 owns medium prognostic value.There was defective of single CA19-9 detection in pancreatic cancer screening,early diagnosis and treatment.The data showed the best positive serum CA19-9 reference values was 155.25U/mL in pancreatic cancer prognosis compared with pancreatisis,which owns higher specificity.There was higher prognosis value of CA19-9 in bile than that in serum.The sensitivity and specification was increased when positive reference value for 1515.2U/mL.So,preoperated bile CA19-9 in pancreatic cancer prognosis was more valuable using ERCP.Further studies showed that the SNPs of PARP-1 Val762Ala(T2444C)was related to incidence in pancreatic cancer.The risk to suffering pancreatic cancer of genotype PARP-1(2444)TC was 2.476 times than that of wide type genotype TT(OR 2.476,95%CI:1.159-5.292,P=0.018).The risk to suffering pancreatic cancer of homozygote CC was 5.778 times than that of wide type TT(OR 5.778,95%CI:2.278-14.657,P<0.000).The risk to suffering pancreatic cancer of allele PARP-1 2444 C was 3.337 times than that of wide type(OR 3.337,95%CI:1.715-6.646,P<0.001).The sensitivity and specificity of single tumor marker was poor for diagnosis of pancreatic cancer,in which,the PARP-1 SNPs mutation owned highest sencitivity(68.18%).The sensitivity of combined detection of serum and bile CA19-9?PARP-1 Val762Ala was highest(93.33%).Immunohistochemistry data showed that the expression of PARP-1 in pancreatic cancer was(80.3%,53/66)significantly higher than that in peri-cancer(34.8%,23/66).The poorer of tumor differentiation,the higher positive of PARP-1.The data also showed that the expression of caspase-3 which could inhibition of PARP-1 was slightly higher in cancer tissue than that in peri-cancer but they did not achieve statistical significance.[Conclusion]The result showed that the CA19-9 in serum and bile was valuable for diagnosis of pancreatic cancer;the SNPs of PARP-1 Val762Ala(T2444C)was related to incidence in pancreatic cancer;the sensitivity of combined detection of serum and bile CA19-9?PARP-1 Val762Ala was highest(93.33%);The SNP of PARP-1 Val762Ala could decreased activity of PARP-1,which may reduce prothetic ability to DNA injury and hence increased the incidence of pancreatic cancer.It demonstrated that SNP of PARP-1 Val762Ala was related to susceptibility of pancreatic cancer.The data could dedicat to pathogenesis and diagnosis and treatment of pancreatic cancer. |
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