| Part one :Expression and Significance of Complement-mediated key components in the pathogenesis of acute fatty liver of pregnancyObjective: To explore the role of humoral immunity in the pathogenesis of AFLP,the expression of the main components of complement in fatty liver of pregnancy were be observed.Methods: All cases of acute fatty liver of pregnancy underwent liver biopsy after the patients’ coagulation function returned to normal, and the normal liver tissue obtained from patients with hepatic hemangiomata were used as control.Immunohistochemistry technology was used to detect the expression of C3 d,C1q,C4 d in liver tissue samples. The correlations between C3 d, C1 q and C4 d expression and the pathological characteristics were analyzed.The result of staining expression was judged using Semi-quantitative analysis.Results1.Our findings indicated that there were significant differences of ALT(220.00±100.48 vs. 43.00±5.18, t=4.309, p=0.008), AST(286.33±138.41 vs.39.83±10.61, t=4.238, p=0.008), ALP(329.33±25.73 vs. 79.67±24.26, t=9.767, p< 0.001), TBIL( 152.50±47.20 vs. 19.00±4.73,t=6.894, p=0.001), hepatic steatosis(F)(1.67±0.82 vs. 0.5±0.00, z=2.331, p=0.020), inflammation grade(G)(1.50±0.55 82 vs. 0)between the AFLP group and the control group.2. The expression of complements: The deposition of C3 d, C1 q and C4 d in liver tissue was observed in two groups. 83%(5/6) cases of sinusoidal endothelial and portal area in AFLP group were strong positive for C3 d and C1 q. Occasionally positive expression was observed within arterial intima. In the control group, 50%(3/6) cases in the control group were positive expression with C3d; and no significant expression with C1 q and C4 d. It displayed that the deposition of C3 d within GC in various degrees, mainly in the denser bundles of collagen fibers. In GC,The expression of C3d(c2=0.375,P=0.540)、C1q(c2=0.375,P=0.540)in AFLP group and the control group was not statistically significant. But in sinusoidal endothelial and portal area, the expression pattern of C3d(c2=5.486,P=0.019) and C1q(c2=5.486,P=0.019)had significantly differences between the two groups.Conclusions: The strongly positive expression of sinusoidal endothelial and portal area in AFLP group with C3 d and C1 q indicates that humoral immune response play a role in the pathogenesis of AFLP.Part two:Expression and Significance of SREBP-1c in the pathogenesis of acute fatty liver of pregnancyObjective: To observe the expression of SREBP-1c in acute fatty liver of pregnancy and to explore the role of endoplasmic reticulum stress in the pathogenesis of AFLP.Method: We had obtained liver tissue from AFLP group and the control group.Immunohistochemistry was used to detect the expression of SREBP-1c in the two groups. The correlation between SREBP-1c expression and the pathological characteristics was analyzed and the result of staining expression was judged using Semi-quantitative analysis.Results1.SREBP-1c was highly expressed with dark brown color in nucleus and some in the cytoplasm. The expression rate of SREBP-1c in the liver tissues was 100%.And mild expression in the cytoplasm of liver cells was found in the control group.Significant difference were observed in SREBP-1c between the two groups.(Z=2.55, p<0.011);2. The expression of SREBP-1 was correlated with hepatic steatosis(rs =0.82, p<0.05) and inflammation grade in the distance(rs=0.949, p=0.004), but not with Necrosis(p >0.05).Conclusions:1. The SREBP-1c highly expresses in nucleus in AFLP groups.2 The expression of SREBP-1c was correlated to hepatic steatosis, and inflammation grade. It is indicative that SREBP-1c may be involved in the important mechanisms for fatty deposition in hepatocytes. |
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