The Synthesis Of Plantinum Nanoclusters And Its Application In Biomedical Research

Malignant tumor, one of the greatest killers leading to death, is a serious threat to human health. Early diagnosis and effective treatment is the most effective way to reduce cancer mortality. In recent years, the continuous development of nanotechnology and the emergence of a large number of new nanomaterials have established a new strategy for the diagnosis and treatment of tumors. For the diagnosis of the tumors, cells and in vivo imaging, nanotechnology with fluorescent nanomaterials has been widely explored, especially the development of nanomaterials with non-toxic, good fluorescence properties and nice tumor sensitivities has attracted more and more attention. Some biocompatible nanomaterials have been also utilized as drug delivery carrier, or as the photosensitizer for photodynamic or photothermal therapy. In this study, fluorescent platinum nanoclusters have been synthesized and explored for bio-imaging and photo-thermotherapy of target tumors. The main research contents of this paper are as follows:1. One-step rapid synthesis of fluorescent platinum nanoclusters for cellular imaging and photothermal treatmentWe have successfully fabricated the fluorescence platinum nanoclusters (PtNCs) with a new simple and green one-step synthesis method through collaborative reduction of ascorbic acid (vitamin C) and glutathione. This PtNCs with a very good biocompatibility, stable fluorescent and targeting property can be used for specificity recognition to tumor cells; what's more, it possess strong lethality to cancer cells when using infrared (IR) irradiation, suggesting that it could potentially be used in early diagnosis and treatment of tumors. This may offer the possibility of the future utilization of PtNCs in fluorescence imaging-guided photothermal therapy to realize the accurate bio-labeling and multimode treatments of target cancers.2. In situ synthesis of platinum nanoclusters for in vivo self-bio-imaging and synergistic effect with porphyrin for photothermal treatment of tumorsWe found that the tumor cells were able to specifically induce the reduction of H2PtCl6 and in situ biosynthesize fluorescent PtNCs but normal cells can't occur, which offered a new method for tumor imaging. When using infrared (IR) irradiation, it showed excellent cancer cell-killing efficiency in combination with porphyrin derivatives for photothermal and photodynamic treatment, thus providing a promising application as bioimaging probes for specific fluorescent self-bio-marking and realize fluorescence imaging-guided photothermal therapy of tumors3. In-situ green synthesis of highly active GSH-capped Pt-Au-Ag-hybrid nanoclusters and study of their catalytic performanceWe firstly prepared PtNCs, then get Pt-Au hybrid nanoclusters, finally fabricated Pt-Au-Ag hybrid nanoclusters through the collaborative reduction and protective effect of ascorbic acid and glutathione. Our studies indicated the process of reduction of 4-NP in a way that is unprecedented. This approach may offer a novel, non-cytotoxicity, efficient catalyst as well as bright fluorescent agent for bio-imaging applications.