The Exploration And Application Of A Novel Sericin Nerve Conduit In Tissue Engineering Field

Partone A Silk Sericin/Silicone Nerve Guidance Conduit Promotes Regeneration of a Transected Sciatic NerveObject: To study the effect of sericinconduitinperipheralnerveinjuryrepairingMethods:We have utilized the pure sericin to prepare a sericin conduit with genipin as a crosslinker. The porosity, pore size, physical and mechanical properties of the sericin conduit have been examined. The Fourier transform infrared(FTIR) spectroscopy analysis was used to examine the secondary structure alterations resulted from genipin crosslinking. The biocompatibility of the sericin conduit have been assessed in vitro. We examined the growth of Schwann cells on the sericin scaffold using CCK-8 assay and the m RNA levels of GDNF and NGF in RSC96 Schwann cells seeded onto the sericin scaffolds. The sericin conduit wrapped with a silicone conduit(sericin/silicone double conduits) is used for bridging repair of a 5mm gap in a rat sciatic nerve transection model. The in vivo repair results are evaluated throughelectrophysiological and histological analyses.Results:The sericin/silicone double conduits achievedfunctional recovery comparable to that of autologous nerve grafting as evidenced by drastically improved nerve function and morphology.Conclusion:The sericin/silicone double conduits achievemorphologic and functional recovery. Importantly,this effect is mainly attributed to the sericin conduit as the siliconeconduit alone only produces marginal functional recovery. Sericin conduit as a novel nerve guide conduit can promote nerve regeneration. The sericin conduit offers an efficient and valuable method torepairing damaged peripheral nerve.Part two glutaraldehyde crosslinked sericin conduit promote long-distance nerve defects regenerationObject:Toexplorethe effect of sericin conduit in long-distance peripheral nerve injury repairingMethods: We fabricate a novel tissue engineering conduit—glutaraldehyde crosslinked sericin conduit(GSC). The physical and mechanical properties of the GSC were examined. And the biocompatibility and bioactivity of GSC were examined by Schwann cells. We applied GSC to repair critical length 15 mm sciatic nerve defects in rats.The in vivo repair results are evaluated through electrophysiological and histological analyses.Results:This GSC have excellent mechanical and physical properties with a high porosity.It also possesses well biological compatibility in vitro, which can support Schwann cells long term survival and up-regulate m RNA level of neurotrophic factors.Both GSC group and autograft group have the same effect on morphology repair at 8weeks.Conclusion: The GSC can promote long-distance never defect regeneration.The GSC may be further functionalized to achieve greaterclinical regeneration outcomes for the repair of larger nerve gapdefects...