Influence Of Barium Titanate Coating Made By The Sol–Gel Method On Casting Cobalt Chromium Alloy On The Cell Proliferation,Cycle And Apoptosis

Objectives The study is to use biological safety testing of of titanium crown the intermediate coating from the point view of new biological cells, by application of MTT assay and flow cytometry test, to observe activity, inhibition of proliferation, the cell cycle and apoptosis of the barium titanate coating extract on L929 cell, to assesse barium titanate coating biosecurity in order to provide experimental basis for clinical application. Methods This study is divided into three parts:1 Sol-gel method of barium titanate coating on L929 cell proliferation. There are four groups in this experiment: barium titanate ceramic coated titanium test piece(group A), conventional titanium porcelain specimens(group B), containing 10% fetal bovine serum RPMI1640 medium for the negative control group(group C), 1% phenol solution as a positive control group(group D), then measured 1d, 3d, 5d absorbance values of each group(OD), calculated the cell relative growth rate(RGR), assessed cells’ cytotoxic levels. 2 Sol-gel preparation of barium titanate coating on L929 cell cycle. Three groups in this experiment: group titanium porcelain specimens containing barium titanate coating, conventional titanium porcelain specimens group, negative control group, each group was detected by flow cytometry to observe the change cell cycle of L929 in 48 h. 3 Sol-gel preparation ofbarium titanate coating on L929 apoptosis. There are three groups in this experiment: group titanium porcelain specimens containing barium titanate coating, conventional titanium porcelain specimen group, negative control group, using Annexin V/PI staining method to make quantitative detection by flow cytometry.Results 1 Preparation of barium titanate coating: Preparation of sol-gel coatings of barium titanate, scanning electron microscope, dense coating, a certain roughness, surface texture, no obvious crack; spectrum analysis: Ti atomic percentage is 10.97%, Ba is 74.22%, O is 7.17%, Si of 5.91%, Al of 1.73%. 2 Two cell growth curve under laboratory conditions. By microscopic observation and cell analysis value, L929 cells were grown in the laboratory of the optimum density is 6×104/ml. 3 MTT test L929 cell proliferation, cytotoxicity, evaluate cells’ cytotoxic levels: in same incubation time, absorbance values of different concentrations of extracts of the positive control group differences were statistically significant(P<0.05), 100% barium titanate coating group absorbance values is greater than the negative control group, the difference was statistically significant(P<0.05); except the positive control group, the absorbance values of each group was increased with the time of culturing extension(P<0.05), comparision absorbance of 25%, 50%, 75% of barium titanate coating groups at 1d with the negative control group, the difference was statistically significant(P<0.05), while at 5d, absorbance of 25%, 50%, 75% barium titanate coating group compared with negative control group was not statistically significant(P>0.05). 4 PI method to measure the results of the cell cycle. G1 phase: compared three groups: barium titanate coating group and conventional titanium porcelain and negative groups, the result was statistically significant difference(P<0.05), compared barium titanate coating group with conventional titanium porcelain bonding group, the difference was not statistically significant(P>0.05); S phase: the comparision result as above. G2 phase: values among the three groups were no significant different(P>0.05).5 PI/Annexin V to detect apoptotic cells results:Barium titanate coating group and conventional group titanium porcelain apoptosis apoptosis compared with the negative group, the difference was statistically significant(P<0.05), compare barium titanate coating titanium porcelain bonding group with conventional group, the difference was no statistically significance(P>0.05).Conclusions 1 Group titanium porcelain coating containing barium titanate compared with conventional titanium porcelain, has no short-term toxicity tests in vitro cytotoxicity ratio, and could promote the role of cell proliferation. 2 The combined group compared with conventional titanium porcelain, no significant difference in cell cycle and apoptosis. 3 It is recommended in clinical use barium titanate ceramic titanium coating to increase the bonding strength, reduce collapse porcelain porcelain crack phenomenon, thereby improving the success rate in repair treatment.