Study On Tocopherol Polyethylene Glycol Modified Hollow Silver Nanoparticles For Antimicrobial Drug-resistance Bacteria Effect

Objective To present a new method using Tocopherol polyethylene glycol 1000 succinate(TPGS1000) as a surfactant to prepare hollow silver nanoparticles(HAgNPs) with good stability, and low toxicity, to prepare Tigecycline loaded HAgNPs which have antibacterial effect to drug resistance bacteria with slow release effect and to study the slow release effect, the bacteria uptake and antibacterial effect to drug resistance bacteria of Tigecycline loaded HAgNPs.Methods A new kind of HAgNPs is prepared which is different from the traditional HAgNPs by changing the surfactant of the synthetic method from sodium citrate(SC) to TPGS1000. After the TIG-loaded HAgNPs are prepared through physical absorption between TIG and HAgNPs, its physical stability, TEM observation, particle size, bacteria uptake and cytotoxicity are investigated. The changing MIC of the standard E.coli strain and drug resistant E.coli strain which are dosed by TIG-loaded HAgNPs through broth dilution method are evaluated. The synergetic antimicrobial effect between space structure, chemical constitution, drug release and bacteria uptake of TIG-loaded HAgNPs and antibacterial effect through drawing time-bactericidal curve to evaluate the antibacterial effect are also studied.Results The particle sizes of SC surfactant HAgNPs, TPGS1000 surfactant HAgNPs and TIG-loaded HAgNPs have been respectively 50 nm, 20 nm and 20 nm. The diameters of TPGS1000 surfactant HAgNPs and TIG-loaded HAgNPs have been uniform distributed and the dispersity is good. The antibacterial effects to standard E.coli and drug resistance E.coli of TIGloaded HAgNPs have been both better than them of TIG and black HAgNPs and there has been a control release effect in the in vitro release experiment of TIG-loaded HAgNPs.Conclusions The safety and redispersibility of TPGS1000 HAgNPs are increased from traditional HAgNPs and they both have bacterial uptake. TIG-loaded HAgNPs has a control release effect and a significant antibacterial effect to drug resistance bacterial.