| Background and objective:Renal ischemia-reperfusion injury is a common clinical pathophysiological process in the acute renal artery block and kidney transplants operation.it is a common complication of renal shock,,renal resection and hydronephrosis.Hypoxia induced factor-1 is a transcription factor which widely exists in mammals and human body in anoxic conditions.it can activate many anoxic reactive gene expression to maintain the survival of cells in ischemia anoxic condition..As the main active subtypes of HIF- 1,the expression of HIF-1 ?in renal ischemia reperfusion tissue have been under the spotlight.Mesenchymal stem cells(MSCs) can through paracrine and endocrine way to relieve acute or chronic renal damage.The microvesicles(MVs) considered is the important medium for intercellular communication each other.Our experiment group early experiments have confirmed that human umbilical cord mesenchymal stem cells(HUMSCs) have protective effect on acute kidney injury,However, its specific mechanism is unclear.This experiment hope to expore the possible mechanism of HIF- 1 ?in the protection fuction of MVs derived from HUMSCs,by observing how MVs derived from the HUMSCs influnce HIF1-? expression in renal ischemia-reperfusion injury of rat.Methods: We randomly divided 30 male adult SD rats into control group(Sham group), ischemia reperfusion group( IRI group),MVs derived from HUMSCs treatment group( MVs group),each group of 10.Adopt the method of right kidney excision and left kidney ischemia to make renal ischemia-reperfusion injury model.Three groups rats were cut off right kidney.For IRI group and MVs group rats,Clamp the left kidney artery 45 mins then loosen the artery clamp,Sham group simulated operation process but not clip left renal artery.MVs group were given 100 ug protein content MVs derived from HUMSCs through the tail vein.Ischemia reperfusion group and Sham integrated by tail vein injection capacity such as saline solution.After 24 h kill all the rats to take blood immediately through the inferior vena cava to detect the contents of SCr and BUN.At the same time we excised the left kidney and took half of the left kidney in 4% paraformaldehyde solution for fixtion, the left half kidney preserve in- 80 refrigerator for PAS stain and detect each ?kidney HIF1-? expression level by Weston- blot.Results:Compared with the Sham group, IRI group kidney pathological damage is heavier,Showing the different degree of glomerular, renal interstitial hyperemia, renal tubular epithelial cell edema hyaline changes in the cell,steatosis, renal tubular lumen protein tube type HIF- 1 ?expression and Scr, BUN, blood levels increased significantly. This difference has statistically significant(P < 0.05). The MVs group compared with the Sham group rats,kidney pathological damage slightly increased,Scr, BUN, blood levels increased slightly, HIF- 1 ?expression descend slightly,This difference has not statistically significant(P > 0.05).Compared with IRI group, kidney pathological damage of MVs group was obviously reduced,Characterized by protein tube type and mild edema of individual specimen.As well as the Blood Scr and BUN were significantly reduced, HIF- 1 ?expression is decreased obviously.This difference has statistically significant(P < 0.05).Conclusion:1.The renal HIF- 1 ?expression increased significantly just when the renal ischemia-reperfusion injury accur MVs derived from HUMSCs can significantly improve renal ischemia reperfusion injury in rats, with renal protective effect MVs derived from HUMSCs can reduce the expression of HIF- 1 ? HIF- 1 may serves as a detection index to evaluate the degree of kidney damage. |
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