)biomechanical Properties Of The Goat Temporomandibular Joint Disc And The Self-assembled Construct

In recent decades,tissue engineering technology research in the field of regenerative medicine has become a landmark of new technologies, and multidisciplinary achieved remarkable results.Traditional tissue engineering include three elements: cells, scaffold and growth factors. However,due to the limitations of the scaffold, the traditional tissue engineering technology in certain areas(such as articular cartilage) is limited. In recent years,Athanasiou put forward scaffoldless tissue engineering,that is,which refers to any platform that does not require cell seeding or adherence within an exogenous,three-dimensional material.Scaffoldless tissue engineering technology is divided into self-organization and the self-assembling process.Studies have shown that the self-assembly technology in cartilage tissue engineering,articular disc tissue engineering research has made gratifying progress.The main research direction of this paper is temporomandibular joint(TMJ) disc tissue engineering.Goats temporomandibular joint disc in the structure and function are high similarity with people, so the goat is chosen as an animal model.Experiment 1, goat TMJ disc different parts were made into standard samples, and the properties of samples were test by using the micro-testing machine.In addition, by studying the microstructure of collagen fibers in the articular disc to explain the difference in mechanical properties, which laid the original foundation for the mechanical properties of self-assembly TMJ disc tissue engineering.Experiment 2, goat TMJ disc self-assembled constructs were successfully constructed by using self-assembly techniques, and relevant biomechanical properties were analyzed. Self-assembled mold was 5mm× 10 mm agarose wells,which provided a closed system for the self-assembly process.The self-assembled construct was formed with a certain biochemical and mechanical properties without the use of external input by the principle of free energy minimization.Finally, the biochemical properties and part of the mechanical properties of the self-assembly constructs were analyzed and compared with the native articular disc.The results obtained, goat articular disc elastic modulus in(2 ~ 3) Mpa,the intermediate disc zone was the largest,then the posterior band, and the anterior band with a minimum. Since the elastic modulus of the self-assembled construct in(500 ~ 970) Kpa, which is lower than that of native articular disc about an order of magnitude. In summary concludes:the self-assembled goatTMJ disc tissue engineering is very practical,and we need to further improve the mechanical properties of self-assembly construct based on the current research results.