| At present, gastric carcinoma is the one of most common malignant tumors in China, the second common malignant tumors in the males only after lung cancer and the third in the females after breast cancer and lung cancer. The mortality is in the third place(male) and in second place(female) respectively. Gastric carcinoma is still one of the leading causes of cancer-related death. The most two important factors influencing the cure rate are the recurrence and metastasis. Therefore, exploring the molecular mechanism of recurrence and metastasis of gastric carcinoma is very important and necessary for improving the cure rate and the condition with gastric carcinoma. Researches have shown that the abnormal expression of Homo Sapiens Enoyl Coenzyme A Hydratase1(ECHS1) may closely correlated with the tumorigenesis, development, invasion and metastasis of hepatocellular carcinoma, rectal cancer, colorectal cancer, breast carcinoma, malignant melanoma and prostate cancer, but the expression and possible effect of ECHS1 has not been completely understood. This study tries to detect the ECHS1 mRNA and protein expression in the patients with gastric carcinoma by RT-PCT and western blot, and to explore the possible effect of ECHS1 in the tumorigenesis, development, invasion and metastasis of gastric carcinoma; therefore it can provide the theoretical evidences for clinical treatment on the gastric carcinoma.Objective:To explore the possible effect of ECHS1 in the tumorigenesis, development, invasion and metastasis of gastric carcinoma, and then to provide the theoretical evidences for clinical treatment on the gastric carcinoma.Methods:The tumor samples and corresponding para-carcinoma normal tissues from one hundred and seventy patients with gastric carcinoma are collected. Firstly, reverse transcription-polymerase chain reaction(RT-PCR) and western blot respectively are used to detect the ECSH1 mRNA and protein expression from 8 of 168 tumor samples and corresponding para-carcinoma normal tissues mentioned above. Secondly, tissue microarray techniques and immunohistochemistry are used to detect the ECHS1 protein expression in the collected samples from all 168 patients with gastric carcinoma. Correlation between ECSH1 expression and sexuality, patient age, serosal invasion, depth of invasion, pathological stage, differentiation, lymph node metastasis, perineural invasion, vascular invasion, tumor location, histological type, tumor size, peritoneal infiltration and metastasis and other clinical pathological are analyzed in this study.Results:1.The result of RT-PCR illustrates that in the tested tumor tissues and para-carcinoma normal tissues from 8 of 168 patients with gastric carcinoma, compared to in the para-cancer normal tissues, the ECHS1 mRNA level in gastric carcinoma tissues is lower, and the difference is statistically significant(p<0.05).2.The result of Western blot illustrates that compared to it the para-cancer normal tissues, the ECHS1 protein level is significantly lower in gastric carcinoma tissues from the 8 of 168 cases and the difference is statistically significant(p<0.05).3. Tissue microarray and immunohistochemistryFirstly, the ECHS1 expression is higher in the para-cancer normal tissues than the tumor tissues and the differences is statistically significant(p<0.05).Secondly, the statistical analysis reveals that the expression of ECHS1 in gastric cancer tissue is not related to sexuality, patients' age, serosal invasion, depth of invasion, perineural invasion, TNM stage, lymph node metastasis, differentiation, organization type, tumor size, peritoneum infiltration and distant metastasis clinical and other pathological parameters(p >0.05).Thirdly, the expression of ECHS1 in carcinoma tissues with vascular invasion is higher than that of non vascular invasion(p=0.019), and the expression level of ECHS1 is closely related to the location tumor. When the tumor occurs to the top 1/3 part of stomach, the expression level is significantly increased(p=0.007).Conclusions: The ECHS1 is correlated with vascular invasion but not the distant metastasis and lymph nodes. What's more, its physiological mechanism is not clear, which needs further study. |
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