| Objective: To establish a rabbit model of traumatic brain hydraulic injury, monitoring brain injury in rabbits injury intracranial pressure(ICP) and mean arterial pressure(MAP)changes, in the calculation of cerebrovascular pressure reactivity index(PRx) change. By cerebrovascular pressure reactivity index(PRx) measurement obtained while maintaining sufficient oxygen for optimal brain cerebral perfusion pressure(CPPopt).Methods: 25 healthy adult New Zealand rabbits, male or female, were randomly divided into experimental group 20, control group 5. Routine monitoring of the arterial pressure, pulse rate. In lateral fluid percussion injury model was established by rabbit brain. Placed side in injury ICP monitoring probe, routine monitoring of ICP values. I n opposite side of injury Epiduralin placed with balloon catheter on the experimental group 20 rabbits, stepped filling up inside the balloon. Matched Group 5 has the balloon without filling water. Until the death of the animal. Continuous monitoring of intracranial pressure, blood pressure and PRx and recorded. At T0, T15, T30, T60, T90 five time points intermittently extracted jugular blood cell, monitored the oxygen partial pressure jugular vein, jugular venous oxygen saturation, hemoglobin and other indicators. After the death of animals, take over the whole brain spinal cord, brain observed gross pathological changes. All data were entered EXCEL2003 and used SAS8.1 for statistical analysis. The method used for the single-factor repeated measures ANOVA and pearson correlation analysis.Results: After the completion of the preparation of the animal model, MAP, ICP increase the volume of the balloon over time, to maintain a relatively constant, and then gradually increased, and gradually decreased up to a certain peak value. CPP changes over time at first remained relatively stable, after gradually reduce. PRx value of MAP, ICP, CPP are closely related. For T01, T25, T55 and T70 four time periods, PRx and CaO2, PRx and CjvO2, PRx and CERO2, PRx and CPP relationship among the four groups were analyzed to obtain r =-0.711, and-0.845-0.773,0.700, four groups were P <0.001. Control group: rabbit brain were milky white color, normal morphology, no swelling and displacement. Light microscopy showed that the brain consistent uniform structure, cell morphology intact, nucleolus clear, cells and perivascular spaces were normal. Experimental group: Brain gross pathology observed the visible surface of the flake parenchymal hemorrhage, brain had purple congestion, brain stem and cerebellum was most obvious. whole brain observed under the Light microscope, the tissue capillaries and vein dilation, blood, red blood congestion in lumen, partial vascular rupture point within the brain parenchyma haemorrhage. Cerebral edema was not significant, and no cell shrinkage and necrosis.Conclusion: Real-time monitoring of intracranial pressure, water injection Epidural balloon establish brain injury intracranial hypertension in animal models, on the one hand to prevent too fast or too high intracranial pressure on brain tissue damage; on the other hand can also be prevent excessive fluctuations in intracranial pressure caused by sharp fluctuations in the animal cycle. This method is reliable tight, easily standardized, with the value of the promotion, provide experimental basis for future clinical work. ICP, CCP and PRx exist relevance, PRx after TBI may help determine the optimal CPP. Cerebral perfusion pressure(CPP) value decreased, can lead to impaired autoregulation of cerebral blood vessels.There is a correlation between PRx and cerebral metabolism. Determination of joint cerebral metabolic has certain reference value, to the indicators of cerebrovascular pressure reactivity for damage assessments. |
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