The Study Of Allogeneic Composite Cell Sheets Of Dental Follicle Stem Cells And Periodontal Ligament Stem Cell Therapy For Periodontal Defects In Beagles

Objective With the rapid development of orthodontic in adult, periodontal problem has been a new big challenge facing orthodontists. The regeneration of periodontal defect is the basis of the orthodontic treatment. We have confirmed that DFSCs/PDLSCs composite cells sheet has better ability of periodontal repair than any other single cell sheet in in vitro. The purpose of this study was to investigate the periodontal healing after application of the composite cell sheets of dDFSCs and dPDLSCs in beagle dogs. Methods Canine DFSCs and PDLSCs were isolated enzymatically and expanded in vitro. We used the dDFSCs, dPDLSCs and the mixed cells of them to make cell sheets. Then, three types of cell sheets were transplanted to dental root surfaces having three-wall periodontal defects in an allogeneic manner, and bone defects were filled with HA/TCP. The periodontal clinical indices were evaluated at the time of 1w, 2w, 4w, 6w, 8w and 12 w after surgery. 12 weeks after surgery, the animals were sacrificed and decalcified specimens were prepared. Healing of the periodontal defects was evaluated histologically and histometrically. Results 1?Canine DFSCs and PDLSCs were isolated enzymatically and expanded in vitro, and their proliferative ability and multipotent differentiation ability have been confirmed. We used the dDFSCs, dPDLSCs and the mixed cells of them to make cell sheets. Then, we proved that dDFSCs and dPDLSCs co-cultured in a ratio of 1:1is optimal for periodontal regeneration. 2?Periodontal clinical examination indicate that the periodontal depth has been restored in the sixth week, sooner than the other two single cell sheets. Histological and histometrical analysis indicate that the periodontal healing of cell sheets groups were superior to the control group, and the mixed cells sheets group was superior to the other two groups( P<0.05). Conclusion The results of this study imply that allogeneic composite cell sheets of dDFSCs and dPDLSCs therapy should prove useful for periodontal regeneration in clinical settings.