The Pharmacological Effects Of Gastrodin On PTSD And MCAO Model

Objectives To investigate the influence of gastrodin(GAS) on the behavior of PTSD model rats and the inflammatory factor level in the hippocampus and its possible mechanism. To investigate the effects of gastrodin(GAS) on the MCAO model rats and the expression of myelin basic protein and neurofilament high molecular weight in the striatum and the IL-1? level in the ischemic brain.Methods The present study was divided into two parts. In the Experiment 1, we observed the behavioral manifestations, the changes of the levels of IL-6 and IL-1?, the expression of induced nitric oxide synthase(i NOS), p38 and phospho-p38(p-p38) in hippocampus in the rats subjected to enhanced single prolonged stress.Rats were randomly divided into Sham group, ESPS group, GAS(L) group, GAS(M) group and GAS(H) group. Sham group were normally fed; ESPS group establishment of ESPS model. Following ESPS,GAS(L) group, GAS(M) group and GAS(H) group were administered orally with gastrodin(50, 100, or 200 mg/kg) or vehicle for 2 weeks.The observation targets include: 1. Behaviors in the open field test, elevated plus maze test. 2. The level of IL-6 and IL-1?. 3. The expression of i NOS, p38 and p-p38.In the Experiment 2, we observed the neurological severity scores and the volume ratio of the cerebral infarction, the changes of the levels of IL-1? in ischemic brain, the expression of myelin basic protein(MBP) and neurofilament high molecular weight(NF-H) in the striatum.Mice were randomly divided into Sham group, MCAO group, GAS group. The mice in Sham group were received fake surgery and saline, and the mice in MCAO and GAS group were exposed to MCAO, and received saline or GAS(100mg/kg)immediately after the operation for 7 days continuous injection.The observation targets include: 1.The neurological severity scores. 2.Triphenyl tetrazolium chloride(TTC) staining. 3. The level of IL-1?. 4. The expression of i NOS and NF-H in the striatum.ResultsThere was a significant decrease in the percentage of time spent exploring the center of the arena, and a significant increase in the immobility time in the ESPS rats compared with those of the sham group. Furthermore, elevated IL-6 and IL-1? levels, the protein expressions of i NOS and p38 and p-p38 in the hippocampus of ESPS rats were significantly increased. Treatment with gastrodin, particularly at higher doses, reversed the aforementioned changes. There was a significant decrease in the neurological severity scores and the volume ratio of the cerebral infarction and elevated IL-1? levels in the ischemic brain, the protein expressions of MBP and NF-H in the hippocampus of MCAO mice were significantly decreased. Treatment with gastrodin reversed the aforementioned changes.Experiment 11. Compared with the Sham group, the rats exposed to ESPS showed an increase in center area entries and percent time spent exploring the center arena in open field test( P < 0.01,P < 0.05); as well as the percent time spent in open arms and the percent number of entries into open arms in elevated plus-maze test( P < 0.01,P < 0.05). the levels of IL-6 and IL-1? increased in hippocampal(P <0.01, P <0.01); the expression of i NOS / ?-actin ratio,and p-p38 / ?-actin ratio and p-p38 / p38 ratio increased in hippocampus( P <0.01, P <0.05, P <0.01).2. Compared with ESPS group, the rats exposed to GSA(H) group showed an decrease in center area entries and percent time spent exploring the center arena in open field test( P < 0.01,P < 0.05); as well as the percent time spent in open arms and the percent number of entries into open arms in elevated plus-maze test(P < 0.01,P < 0.01). The levels of IL-6 and IL-1? decreased in hippocampal( P <0.05, P <0.05); the expression of i NOS / ?-actin ratio, and p-p38 / p38 ratio decreased in hippocampus( P <0.01, P<0.05).3. Compared with ESPS group, the rats exposed to GSA(M) group showed an decrease in center area entries and percent time spent exploring the center arena in open field test( P < 0.05,P < 0.01); as well as the percent time spent in open arms and the percent number of entries into open arms in elevated plus-maze test( P < 0.01,P < 0.01). The expression of i NOS / ?-actin ratio, and p-p38 / p38 ratio decreased in hippocampus( P <0.05, P <0.05).Experiment 21. The neurological score and infarct volume of Sham group is ozero,compared with MCAO group, the mices exposed to GAS group the neurological score and infarct volume was significantly lower(P <0.05).2. Compared with the Sham group, the mices exposed to MCAO group the integral optical density of MBP and NF-H was significantly lower(P <0.05), the level of IL-1? in the ischemic brain was significant higher(P <0.05).3. Compared with the MCAO group, the mices exposed to GAS group show a increase in neurological score(P <0.05), and an decrease in infarct volume(P <0.05), The integral optical density of MBP and NF-H was significantly higher in the striatum(P<0.05), the level of IL-1?was significant lower in the ischemic brain(P <0.05).ConclusionThese results indicate that gastrodin possesses anxiolytic effect and may be an effective herbal preparation for the treatment of PTSD. At the same time Gastrodin could improve neural function of mice after middle cerebral artery occlusion, and it may play an important role in protecting myelin and nerve fibers of striatum.