E2F1 Reduces Wound Healing By Suppressing Neovascularization Through Regulating VGEF Expression

Introduction: E2F1 is one important member of E2 F family which play a central role in cell cycle control and therefore provide an ideal target for therapeutic modulation of vascular growth. And vascular growth is an important part in the skin wound healing. We thus seek out to investigate the functional significance of E2F1 in the process of skin wound healing.Methods: We design our experiment with E2F1 knockout mice and WT mice. After anesthetized, a stetile 10 mm punch biopsy tool was used to outline a circle pattern for the wounds on one side of the dorsum. Digital photographs were taken on the day of surgery,3 days,7 days,10 days,14 days after surgery, and wound area was analyzed by tracing the wound margin with Image Pro Analysis software. 7 days or 14 days after surgery, the wound were excised, bisected, and fixed in 4% polyformaldehyde, and underwent histological processing with HE and Masson. Western blot analysis of VEGF protein expression level and real time PCR analysis of VEGF m RNA expression level from the excised wound tissue were also carried out.Results: In this experiment, E2F1 knockout mice display a reduced wound size and have faster wound healing rate; And the dermal thickness and collagen amount are increased in the skin wound of E2F1 knockout mice; And E2F1-/-mice exhibit an enhanced neovascularization in the border zone of skin wound; E2F1 knockout alsolead to increase VEGF expression in the skin wound.Conclusions: We found that E2F1 knockout mice have faster wound healing function recovery.