UQCRC1 Promotes Metastasis Of Cervical Cancer Cell SiHa Via Affecting The Activity Of Mitochondrial Complex Ⅲ

Objective: In this study, we tried to investigate the status of UQCRC1 in patients with human cervical cancer and examine the role of UQCRC1 in metastasisMethods: An analysis of the immunostaining patterns of UQCRC1 in normal cervical tissue and cervical cancer samples was performed. Small-interferon RNA was used to inhibit the expression of UQCRC1. The morphological images were observed and filmed via light microscopy. Transwell assay was used to examine the ability of metastasis in SiHa cells. The activity of complex III was assayed with Mitochondrial Complex III Activity Detection kit and Cellular ATP content was determined using ATP assay kit. Real-time PCR were used to determine the m RNA expression. The related protein expressions were measured by western blot analysis.Results: UQCRC1 expression was significantly elevated in cervical carcinomas as compared to normal tissues. Suppression UQCRC1 using si RNA caused morphological changes, according with characteristics of the mesenchymal–epithelial transition(MET). This switch accelerated cancer cell metastasis in vitro assays. Notably, UQCRC1 knockdown cells exhibited severe defects in Complex III activity and cellular ATP production, finally, AMPK was activated.Conclusion: UQCRC1, accumulating in cervical cancer tissues, increase the activity of mitochondrial complex III and ATP production, inhibits AMPK activity, then promotes metastasis in SiHa cells.