Research On The Inhibition Effect Of Intra-cicatrix Injection Of ADSCs Using A Rabbit Ear Hypertrophic Scar Model

Objective Investigate the inhibition effect and mechanism of intra-cicatrix injection of adipose derived stem cells on hypertrophic scar using a rabbit ear scar model.Method Twelve New Zealand white rabbits used for creating scars were randomly divided into 3 groups. 2 weeks after the surgery, all the right ears were injected DMEM as internal control while left ears of group 1,2 were injected ADSCs, ADSCs-CM respectively. Left ears in the third group were remained untouched. Photos and ultrasonography were taken before and 1,2,3 weeks after the injection. Histochemical examination and genetic detection were done when the rabbits were sacrificed for offering scar tissue 3 weeks later.Results All wounds are re-epithelized 2 weeks after the surgery. Wounds injected DMEM and untouched gradually became thick, red and stiff which are the symptoms of hypertrophic scars while ADSCs and ADSCs-CM injected ones show no sign of hypertrophic scar. HE and Masson staining show collagen deposit and irregularly arrangement in the DMEM injection and untouched scars while collagen deposit is much less and well arranged in both ADSCs and ADSCs-CM treated ones. Genetic detection shows lower expression of ?-SMA?Collagen ? in ADSCs and ADSCs-CM injection scars than that in DMEM and untreated ones. Dil label staining shows larger amount of ADSCs in the scar tissue.Conclusion Intra-cicatrix injection of ADSCs can inhibit hypertrophic scar through ameliorating collagen deposit by down-regulating the expression of ?-SMA?Collagen ?.