| ObjectiveTo establish chronic HUA model in rats, observe the effect of different dose of SP on serum UA and lipid levels of normal rats and HUA rats, explore the possible mechanism of SP on HUA and MS. MethodMale SD rats were divided into three groups: control group(CON), PO group(250 mg/kg bw×d PO), PO combined with YE group(250 mg/kg bw×d PO and 1.5g YE, PO+YE) to establish chronic HUA rat models. UA enzyme colorimetry were used to detect SUA level. Glycerol phosphate oxidase-peroxidase enzymatic were used to detect TG, TC level. Inosine acid oxidase method to detect creatinine CRE level. The rate of enzyme coupling method were used to detect BUN level. The HDL and LDL levels examined by direct method. The blood pressure level detected by noninvasive tail set method. The activity of XOD an ADA examined by test kits. HE staining was used to observe the renal tissue morphology.SD rat were treated with differet concentration of SP and induced chronic HUA rat models.The purine contents in feeds were examined by HPLC. SD rats were invided into four groups:deficiant SP group(22% CA,SP-D),low SP group(11%SP,SP-L), medium SP group(22%SP, SP-M), high SP group(44%SP, SP-H) treated with different SP 4 week. Indirect blood pressure was measured in conscious rats by the standard tail-cuff methods. The serum were collected to detect the biochemical levels, the activity of XOD and ADA. Then half rats of SP-M group were used as control, the other four groups estamblished HUA rat models and treated with differet feeds. After 4 week intervention, the serum were collected to test the biochemical levels, the activity of XOD and ADA. The pathological changes and ultrastructures in renal tissue were examined by opticalor electron microscopy. The main specific biomarkers were confirmed by NMR and PCA. ResultCompared with control, SUA level of two intervention groups started to elevate and reach a high level at 4,6 week. Compared with control, the level of TG increased at 6 week(P<0.05). BP and rat renal histology of model groups weren't detected abnormal.After 4 week treat with different concentration SP, compared with SP-D group and SP-L group, blood test revealed the SUA levels in SP-M group and SP-H group were increased(P<0.05). The activity test of ADA showed that it was improved with the increasing of SP concentration(P<0.05). Compared with the other groups, TG concentrations of SP-H group decreased(P<0.05).After the HUA rat models established successfully,the test of SUA showed that, compared with other model groups, SP-M group decreased(P<0.05). GLU level test showed that compared with control, GLU concentration in SP-D group and SP-L group increased(P<0.05).TC level test showed that, compared with SP-D group, TC level of SP-M group and SP-H group were decreased(P<0.05). BUN level test showed that compared with control, the BUN concentration of SP-D group, SP-L group and SP-H group increased(P<0.05). The activity test of XOD showed that compared with SP-D group, the activity of XOD of SP groups decreased(P<0.05).Metabolic profile changes were found in serum of HUA rats treated with different SP. They are lipdid, LDL, HDL, creatine, pyruvic acid and other 11 kinds of differences metabolites. ConclusionHUA rat model were successfully established and founded that SP can reduced SUA concentration and delay progress of MS in HUA rat, medium concentration had the suitable effect.The mechanism maybe related to reducing activity of XOD and ADA, the enzymes in UA metabolism, and improve metabolic products involving metabolic pathway of GLU and lipid. |
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