Association Of Visfatin-1535?-4689?-423 Genepolymorphisms With Metabolic Syndrome Of Muslim And Han People In Ningxia

Objective In recent years, the study found that the visfatin closely related to metabolic diseases such as central obesity, diabetes, high blood pressure, metabolic syndrome, et.al. But its effect on the disease mechanism is not fully clear. Our study analysis the association with the visfatin-1535 C>T?-4689 G>T?-423 A>G gene polymorphism and metabolic syndrome and we explored the visfatin effects on the disease from the perspective of genetic susceptibility. That to provide a scientific basis for prevention of the metabolic syndrome.Methods In this case-control study, according to the definition of MS proposed by the National Cholesterol Education Program —Adult Treatment Panel III, and modified by American Heart Association(ATPIII-modified)(2002), 376 unrelated patients with MS and 408 unrelated healthy people among inpatient the regular health checkup people were selected randomly from January 2012 to June 2013 at Ningxia Medical University General Hospital and Wuzhong City People Hospital. Inpatients and out-patients were in both the case and control group. The study patients' average age was 50.62±7.04 years(188 males, 188 females) and controls' average age was 51.89±6.32 years(206 males, 202 females). We used questionnaire, physical examination and laboratory biochemical index to collect the basic data of the objects and used sequenom time-of-flight mass spectrometry to detect the visfatin-1535 C>T,-4689 G>T,-423 A>G gene polymorphism.Results The average level WC, BMI, WHR, SBP, DBP, WBC, FPG, TG, TC, LDL-C, AST, ALT and UA in MS group were higher than those in control group(p< 0.05), only the level of HDL-C was lower in MS group(p< 0.05).Visfatin-1535 G>A and-4689 G>T in various clinical indicators did not differ between different genotypes(p> 0.05). In-423 T>C loci, the levels of TG, AST and ALT in genotype TC were higher than those in genotype TT(p< 0.05). The genotype distribution at-1535 G>A in MS and control group had difference(p< 0.05), the allele distribution also had difference(p< 0.05), and people with homozygous genotype AA had the MS risk was 1.64 times as people with homozygous genotype GG, the difference was statistically significant(p< 0.05). The distribution of genotype at-4689 G>T had no difference when compared with MS and control group(p> 0.05), but the allele distribution was different statistically(p< 0.05). The frequency distribution of genotype and allele between control group and MS group had no statistical significance(p> 0.05). The frequency distribution of genotype and allele between MS with no fat group and MS with fat group had no statistical significance(p> 0.05). The serum WBC and TNF alpha expression had no difference among different genotypes of the three locus(p> 0.05) and the levels of serum WBC and TNF alpha had no interaction with-1535 C>T?-4689 G>T?-423 A>G gene polymorphism. The three genetic locus of-1535 C>T,-4689 G>T,-423 A>G had strong linkage disequilibrium, and the frequency of haploid TGA constituted by the three genetic locus in MS group was higher than the control group(51.1% VS 44.9%), it could increase the risk of deeloping MS(OR > 1, 95 % CI 1.048-1.564). But the frequency of haploid TTG constituted by the three genetic locus in MS group was lower than the control group(6.8% VS 9.9%), and this could reduce the risk of deeloping MS(OR < 1, 95 % CI 0.463-0.961). The distribution of genotype and allele of-1535 C>T,-4689 G>T,-423 A>G between the Hui and Han had no difference(p> 0.05).Conclusion The vifatin genetic loci-1535 G > A(rs61330082) associated with the occurrence of MS and homozygous mutation genotype AA can increase the risk of MS. The vifatin genetic loci-4689 G>T(rs2110385) had no associated with the occurrence of MS. The vifatin genetic loci-423 A>G(rs1319501) associated with serum TG, AST, ALT expression level, had no relevance with MS. All the gene polymorphism of three genetic locus had no interaction with WBC and TNF-?. The three genetic locus of-1535 C>T,-4689 G>T,-423 A>G were in strong linkage disequilibrium state. The haploid TGA constituted by the three genetic locus positively correlated with the incidence of MS, and it could increase the risk for MS, but the haploid TTG could reduce the risk of MS. The distribution of genotype and allele of-1535 C>T,-4689 G>T,-423 A>G between the Hui and Han had no difference, and this three genetic locus had no correlation with the MS in Hui and Han nationality.