The Preparation And Transdermal Studies Of Ceramide ?B Nanoliposome And Glabridin Nanoliposome

Ceramide IIIB is a new skin care products ingredient of moisturizing, which supports the renewal of the stratum corneum, and repairs the natural protective layer for against moisture loss. These are therefore particularly suitable for long term protection and repair of sensitive and dry skin. But it is difficult for ceramide IIIB to apply in the skin cream due to its low-solubility, and crystal formation. Glabridin as a polyphenolic flavonoid of licorice extract has been known for its beneficial effects on the skin such as antioxidant properties, skin-lightening effect. But the addition in skin care products is too low to function effectively for its poor water solubility. In this study, ceramide IIIB and glabridin nanoliposomes were prepared by high pressure homogenization technique, which improved their solubility, stability and transdermal absorption significantly. The main results are as follows.(1) The high pressure homogenization technique and response surface methodology were used for preparing the ceramide IIIB nanoliposome and optimizing its prescription, respectively. The size of ceramide IIIB nanoliposome was 71.7 nm, PDI was 0.214, zeta potential was-23.3 mV, the drug loading(DL) and encapsulation efficacy(EE) of ceramide IIIB was 2.3% and 95.9%, respectively. The results of stability study show that ceramide IIIB nanoliposome was stable when it was stored for 3 months, and the cream containing ceramide IIIB nanoliposome remain stable without any crystal formation.(2) In vitro drug release behavior and transdermal property of ceramide IIIB nanoliposome were studied by dialysis bags and Franz diffusion cells, respectively. Results show that the cumulative drug releasing and permeation of ceramide IIIB nanoliposome and its cream were all higher than that of ceramide IIIB cream. The skin retention of ceramide IIIB nanoliposomes cream in 24 h was 7 times than that of ceramide IIIB cream which with the same content of ceramide IIIB. The results indicated that ceramide IIIB nanoliposome can increase drug release and exhibited a significant epidermal targeting effect.(3) Short-term efficacy was performed on mice skin before and after dosing 40 days, the moisturizing effect of ceramide IIIB nanoliposome was evaluated. Results show that the water binding capacity of mice skin with ceramide IIIB nanoliposome applying is increased, and the total content of ceramides in mice skin applied with ceramide IIIB nanoliposome was higher than control group.(4) Glabridin nanoliposome was prepared through high pressure homogenization technique, which with properties as 70.5 nm on size, 0.231 on PDI,-30.0 mv on zeta potential, 1.16% and 73.2% on drug loading and encapsulation efficacy, respectively.(5) The in vitro drug release behavior and transdermal property of glabridin nanoliposome and nanostructured lipid carriers(NLC) were studied by dialysis bags and Franz diffusion cells, respectively. Results show that the release rate and release quantity of glabridin nanoliposome were lower than that of glabridin NLC, but the skin permeation and retention were higher than the latter significantly, indicating that the glabridin nanoliposome can enhance the transdermal absorption of glabridin.(6) The changes of skin condition and melanin particles, SOD activity, MDA content in mice after dosing 40 days were investigated. Results show that the SOD activity of mice skin applied with glabridin nanoliposome was higher than that of the control group, indicating that the glabridin nanoliposome has the potential of antioxidant properties.