Allologous Transplantation Of Mice Satellite Cell Accelerates Injured Muscle Repair

Skeletal muscle injury and the loss of muscle mass caused by direct strain and contusion in sports or metabolic dysfunction would impact our daily behavior. Skeletal muscle very efficient regenerative capacity is supported by satellite cell. However, a large muscle injury cannot restore normal function by a few satellite cells and will be form scar. Many studies have shown that transplanted satellite cell can improve muscle repair,but efficiency was limited by immune reaction and survival rate.Our previous study has confirmed the repair of mouse skeletal muscle injected CTX was accelerated by transplanted allologous satellite cell. Then, we detect the marker genes expression which relate to myogenesis, Notch signaling pathway and inflammation by RT-PCR and Western-blot. The main results were as follows.1.The m RNAs level of Myo D ? Pax7 ? P21 ? Myo G(myogenin), which regulate myogenesis, reached the peak at day 3 after injected satellite cell. The m RNA levels were up-regulate and appeared at an early stage compare with injected PBS as a control. The protein levels of these genes also have a similar result. The m RNA and protein levels of MYH1 also were up-regulate and appeared at an early stage compare with injected PBS. These result indicated that injected satellite cell after muscle injury could accelerate repair.2. The m RNA and protein level of Notch1 reached the peak at day 7 after injected satellite cell and PBS, but the Notch1 expression in injected satellite cell higher than PBS. And we detected the m RNA level of Jagged1(Notch ligands) and found that also reached the peak at day 7. The result illustrated the up-regulation of Jagged1-Notch1 pathway after injected satellite cell compare with PBS. However, the m RNA level of Hes1 after injected satellite cell day3 lowers than injected PBS, and day 14 higher than injected PBS indicating that other Notch receptors impact Hes1 expression.3. The m RNA level of pro-inflammatory factor TNF? and anti-inflammatory factor IL-10 after injected satellite cell reached the peak earlier and higher than injected PBS. At the protein level, TNF? had little change, and IL-10 reached the peak at an early stage after injected satellite than injected PBS. This result suggested that inflammation also was accelerated.This research confirmed that exogenous transplantation of satellite cell enhanced activated capacity of satellite cell and accelerated the inflammation reaction and impacted the Notch signaling pathway. So these programs may make a key contribution to muscle repair.