Association Of DCBLD1 And TP63 Polymorphisms With Susceptibility Of Lung Cancer In Chinese Population

Objective:Lung cancer is one of the major causes of the present human cancer deaths,which are listed in the incidence of various tumors of the first in North America,Europe,and the morbidity and mortality rates trend to increase.This trend is particularly evident in China.Although in recent years,continuous improvement and progress of treatment,the survival rate has increased,but the mortality rate remains high.The fundamental reason is the lack of effective lung cancer screening and early diagnosis and treatment methods.application of modern molecular biology techniques,genetic screening of lung cancer susceptibility genes and molecular markers in lung cancer,lung cancer will become an important means of screening susceptible populations.Single nucleotide polymorphisms(single nucleotide polymorphism,SNP)and susceptibility to lung cancer lung cancer may be susceptible to effective screening and early diagnosis and treatment of lung cancer to help.This study selected rs9387478(A> C)sites,and rs10937405(C> T)TP63 gene loci DCBLD1 gene on its case-single nucleotide polymorphisms and lung cancer susceptibility-control study to illustrate the correlation between them.Methods:Through the First Affiliated Hospital of Soochow University lung cancer patients and healthy people examination,We conducted a case-control study.Peripheral blood samples and specimens collected information through sample screening and genomic DNA extraction ultimately associated with these study 704 cases of lung cancer patients and 900 healthy control subjects to participate.Using high-resolution melting curve(high resolution melting,HRM)technology for genotyping;use SPSS19.0 statistical analysis software for genotyping data were analyzed: Genotype analysis using the chi-square test cases and controls and the allelic gene frequency distribution of differences,and statistical analysis using Logistic relevance of these polymorphisms and lung cancer susceptibility loci,with OR value and 95% Cl indicate lung cancer risk,and according to smoking,drinking,age,gender stratified analysis.Results:rs9387478 locus in heterozygous genotype compared to model its AC AA genotype in smoker group can significantly increase the risk of lung cancer(smoking group: OR = 1.83,95% CI: 1.19-2.83,P = 0.006).In addition,under the dominant model of AC genotype and CC genotype compared to the AA genotype in smoker group can significantly increase the risk of lung cancer(smoking group: OR = 1.69,95% CI: 1.12-2.55,P = 0.013).rs10937405 locus in homozygous genotype TT model compared to the CC genotype in the female group can significantly reduce the risk of lung cancer(female group: OR = 0.40,95% CI : 0.18-0.89,P = 0.034).Conclusions:Rs9387478 site and rs10937405 site are not association with the incidence of lung cancer.rs9387478 locus in heterozygous genotype compared to model its AC AA genotype in smoker group can significantly increase the risk of lung cancer.In addition,under the dominant model of AC genotype and CC genotype compared to the AA genotype in smoker group can significantly increase the risk of lung cancer.rs10937405 locus in homozygous genotype TT model compared to the CC genotype in the female group can significantly reduce the risk of lung cancer.This laid the foundation for further explore the mechanism of lung function as well as the gene.