The Preparation Of Carboxymethyl-pachyman Component And Its Antioxidant Effect

Poria cocos as fungal dry sclerotia, its main composition is Indian buead polysaccharide, accounts for about 90% of the dry weight of the left and right sides, after chemical modification of carboxymethyl tuckahoe polysaccharide has been proved to have anti-tumor medicine value.Based on the optimization of poria cocos polysaccharide extraction process and chemical modification, groping has antitumor effect to concrete components and carboxymethyl tuckahoe polysaccharide of exploitation and utilization value.The main results were as follows:1. Through to the NaOH concentration, extraction temperature, extraction time and solid-liquid ratio four factors in the orthogonal experiment, the optimal process of poria cocos polysaccharide extraction is:0.7 mol/L NaOH concentration, extraction temperature 5℃, extraction time,8 h, material liquid is rising.Poria cocos polysaccharide extraction yield can reach 73.62%.2. Through to the concentration of isopropyl alcohol. NaOH/chloroacetic acid mole ratio, etherification temperature three factors orthogonal experiment was carried out, for the preparation of high substitution degree of carboxymethyl poria cocos polysaccharide of optimal process is:isopropyl alcohol concentration 75%, NaOH/chloroacetic acid molar ratio of 2.5:1, etherification temperature 85℃.Carboxymethyl tuckahoe polysaccharide substitution degree can reach 1.552.3. Indian buead polysaccharide water solubility with an average of 0.041 mg/mL, water solubility and carboxymethylated poria cocos polysaccharide with an average of 0.103 mg/mL, carboxymethyl of poria cocos polysaccharide of water-soluble is about 2.5 times of poria cocos.B ChunChun sink after using different concentration, different concentration of alkali extraction, different reaction time extraction respectively CMP1-CMP 11 11 components such as carboxymethyl tuckahoe polysaccharide.4. By the method of GPC-RI-MALS coupled CMP1-CMP 11 is studied for the molecular weight and molecular weight distribution:in processing components, CMP9 components Mw value maximum,2.990 x 105 Da;Minimum value CMP8 components of Mw.1.683 x 105 Da;CMP9 components dispersion coefficient is the largest. 1.997;Minimum CMP11 components dispersion coefficient, 1.324.CMP1 CMP 11 rotating radius are analyzed, distribution fitting of the component molecules in solution configuration diagram to get the slope, get the components in the solvent distribution differences:the CMP2 and CMP3 for random linear cluster distribution;CMP1, CMP5. CMP9, CMP10, CMP11 for similar ball distribution;CMP4, CMP6, CMP7, CMP8 for spherical distribution.5, By reducing power, hydroxyl free radical ability, remove superoxide free radical ability:the antioxidant effect of preliminary inquiry components all have certain antioxidant capacity, including 0.8 mol/L NaOH concentration, reaction time 8 h, alcohol concentration 80% conditional processing components antioxidant capacity than other components.The results show that the antioxidant activity and molecular space structure is a typical structure-activity relationship:the distribution of the random string, CMP components antioxidant capacity has no obvious regularity, the CMP components antioxidant capacity of spherical distribution is stronger than the class ball distribution of CMP components.