Research Of Capillary Driven Microfluidic Chips And Its Applications In Biomedical Field

Microfluidic chip is the technology of integrating the basic functions of the biological and chemical laboratories to a tiny chip.This technology has achieved rapid development during the past 30 years and has been widely used in environment monitoring,food testing,biochemical analysis,pharmaceutical engineering and some other areas.Compared with traditional microfluidic chips made by glass or quartz,paper-based microfluidic chips enjoy many advantages like better biological compatibility,lower cost and no need of pump or valve,which enables a considerable application prospect for paper-based microfluidics in field testing and clinical medical diagnosis.On the basis of depth study of microfluidic chips related theories and understanding of the present research situation of microfluidic chips,this paper proposed a new method to manufacture a capillary driven microfluidic chip.Then an automatic and high-throughput fluid feed device special for paper-based microfluidic chips is developed.A series of biomedical experiments is carried out to verify the feasibility of the device.The specific work included in this paper is as follows:First,a microfluidic chip manufacturing method based on flash foam stamp lithography is proposed.This method is low-cost and simple operation.This paper then researches and analyses the feasibility of this method and the performance of the chips by testing many parameters such as the contact angle,resolution and flow rate.Furthermore,this paper also carries out a method to fabricate three dimensional paper-based microfluidic chips by PDMS curing.Secondly,this paper independently designs and manufactures an automatic and high-throughput fluid feed device.This device can solve the weakness of capillary driven microfluidic chip which can’t achieve continuous perfusion.A fluid feed function module and a liquid absorption module make up the whole device.In addition,the main parts of this device are fabricated by FDM 3D printing.In this way,the device can be personalized customization according to specific application requirements.In this paper,a concentration gradient experiment is carried out to analyze the feasibility of the device.Finally,combined the capillary driven microfluidic chips with the automatic high-throughput fluid feed device,two application experiments in biomedical field are carried out in this paper.The two experiments are dynamic cell culture experiment on chips and multi-concentration drug screening experiment.Cells viability on paper-based microfluidic chips is analyzed by cell dyeing.Cell proliferation on chips is analyzed by a CCK-8 test.By these analysis,the advantages of dynamic cell culture on chips are evaluated qualitatively and quantitatively.Further,in this paper,a multi-concentration and dynamic drug screening experiment for the cells of human breast cancer is successfully carried out.These experiments lay a solid foundation for the new culture mode for later applications in the biomedical field.