The Study Of The Electrophysiological Mechanism On Temporal Lobe Epilepsy Rats' Cognitive Functional Changes

Epilepsy is one of the common intractable diseases in the nervous system,and the temporal lobe epilepsy is the main type of epilepsy.Repeated seizures may lead to cognitive dysfunction of the patients.But so far,the neural mechanism of cognitive impairment caused by epilepsy has not been fully elucidated,which has attracted much attention on both clinical and fundamental scientific investigations.The rat model of temporal lobe epilepsy induced by pilocarpine is considered to be a highly suitable simulation of the behavioral and pathological damage of patients with clinical temporal lobe epilepsy.Thus it is widely used in the study of the pathogenesis and cognitive impairment of temporal lobe epilepsy.In this work,based on the model of temporal lobe epilepsy in rats,the neural mechanism of hippocampus and prefrontal cortex in cognitive function of temporal lobe epilepsy rats has been studied by behavioral and multichannel neurophysiological techniques,which can be expected to provide experimental information and data for further understanding the knowledge of cognitive impairment of epilepsy.The main results of this thesis are as follows:1.Behavior studies indicated that spatial memory involved the changes of the object's position and non-spatial memory involved the changes of object's shapes have been impaired.2.The power spectrum of the EEG signal analysis showed that the power spectrum of the Delta,Beta,Theta,Low Gamma and High Gamma band were significantly reduced for the space task of the epilepsy rats.Compared to the normal rats,the power spectrum of the Delta,Beta,Theta,Low Gamma and High Gamma bands of the hippocampus and the prefrontal cortex is significantly reduced for the non-spatial task of the epilepsy rats.3.The coherence analysis found that the coherence between hippocampus and prefrontal cortex of the epileptic rats was significantly decreased in non-spatial tasks.It is suggested that the functional connection between the specific brain regions of the epileptic rats is damaged in the memory task of cognitive function.4.With the calculation of the rate of the neuronal discharges,the discharge rate of hippocampus and prefrontal neurons for both the epileptic rats and normal rats was found to be significantly higher than those in baseline,and the discharge rate of neurons in prefrontal cortex was higher than that in baseline.The hippocampus was found to be significantly higher in the space memory task.In the state of epilepsy,the prefrontal cortex of the rats in the non-spatial memory task was found to be significantly reduced,hippocampus in space and non-spatial memory task was found to be significantly reduced.In the cognitive process of memory,it involved neuronal activity,which indicated that the cognitive function of epilepsy was subject to certain damage.