Structure And Appraise A Hyaluronic Acid Hydrogel Scaffold Modified With Nogo-66 Receptor Antibody And Poly-l-lysine In Order To Treat Spinal Cord Injury

Object Neurons is one of the cells who have the most poor regeneration ability in the human body.The nervous system is one of the most important systems in the human body.The injury of Neuronal cell often leads to serious irreversible consequences.To realize the repair and regeneration of the neurons,even functional recovery,after the spinal cord injury is the key point.We provide a new idea for the treatment of spinal cord injury by using stem cell technologies and tissue engineering technologies.The key of the treatment of spinal cord injury include cells,three-dimensional structure,Microenvironment.We created the hyaluronic acid hydrogel scaffold by HA who is the main Extracellular matrix in neurons.In order to culture the endothelial progenitor cells(EPCs)transfected with ?-NGF and a newly created hyaluronic acid hydrogel scaffold modified with Nogo-66 receptor antibody and poly-l-lysine,create the hyaluronic acid hydrogel scaffold and study the feasibility of the hyaluronic acid hydrogel scaffold as the tissue engineering material for the repair of spinal cord injury.To evaluate the biocompatibility of the EPCs transfected with P-NGF and a newly created hyaluronic acid hydrogel scaffold modified with Nogo-66 receptor antibody and poly-l-lysine;studying the feasibility of the hyaluronic acid hydrogel scaffold as the tissue engineering material for the repair of spinal cord injury to provide the basis for the treatment of spinal cord injury by using stem cell technologies and tissue engineering technologies.Methods The Nogo-66 receptor antibodies and poly-1-lysine were grafted to the porous hyaluronic acid hydrogel scaffold which was produced by freeze drying method.In vitro study,the EPCs were isolated from wistar rats,then the ?-NGF was transfected to EPCs by recombinant adenovirus.EPCs were cultured on the hyaluronic acid hydrogel scaffold,and the internal structure of the hyaluronic acid hydrogel scaffold was observed by scanning electron microscope.The growth of the EPCs cultured on the scaffold was determined by the cell vitality detection(CCK-8)and HE.The cytotoxicity of hyaluronic acid hydrogel scaffold was detected by the quantity assay for the expression of lactate dehydrogenase(LDH).The expression of?-NGF was detected by Real Time-PCR,Western blot and enzyme-linked immunosorbent(ELISA)assays in vitro.Result The EPCs which were cultured growing well.EPCs transfected with?-NGF could express the P-NGF.The poriferous three-dimensional structure of hyaluronic acid hydrogel scaffold was observed with scanning electron microscopy.The cytotoxicity of HA hydrogels stents is no difference between the culture plate.EPCs grew well on the scaffolds,and the expression of ?-NGF in experimental group was obviously higher than the negative control group and EPCs groupConclusion The EPCs transfected with ?-NGF grow well and could express the?-NGF.It could be the ideal cell.EPCs and the newly created hyaluronic acid hydrogel scaffold modified with nogo-66 receptor antibody and poly-l-lysine have a good biocompatibility,and EPCS can continuously express(3-NGF in vitro.The newly created hyaluronic acid hydrogel scaffold might be used in the repairing the spinal cord injury as an optimal biomaterial for tissue engineering in the future.