The Expression And Clinical Significance Of RUNX3 In Renal Carcinoma

BackgroundRenal cancer cell carcinoma is the second most common malignant tumor in adult urinary system.In our country the morbidity and mortality accounted for 2%of the whole body malignant tumor.Surgical resection is the main treatment of early regional kidney cancer,however due to the characteristics that the advanced metastatic kidney cancer is not sensitive to chemotherapy and radiotherapy,the prognosis of advanced kidney cancer is not optimistic.Biological immunotherapy and targeted therapy currently become the main treatment of advanced kidney cancer,although significant progress had been made in clinical effect,most patients can not get complete remission rate and tumor progression-free survive,therefore,developing new target needs to be solved urgently.Anthropogenic RUNT related transcription factor 3(RUNX3)is the core of the RUNT family members.As a signal downstream factor of TGF-beta signal pathway,RUNX3 can promote tumor cell apoptosis so as to regulate tumor cell proliferation,death,angiogenesis and invasion.At present a large number of studies have shown that RUNX3 gene is a tumor suppressor gene,it's inactivation,mutation and loss has a close relationship with the occurrence of stomach cancer,colon cancer,breast cancer,pancreatic cancer and other tumors.However,there is some article reporting that RUNX3 is highly expressed in some part of head and neck cancer,indicating that RUNX3 may be carcinogenic.ObjectiveBased on the above situation,this paper preliminary studied the different expression of RUNX3 between kidney cancer and cancer adjacent tissues,and explored the mechanism through the literature review.We also studied the relationship between the expression of RUNX3 and clinical prognosis.Method1.We collected 20 cases of kidney cancer and relative para-neoplastic tissue between January 2014 to January 2014 in QI LU HOSPITAI of SHANDONG UNIVERSITY.All of the cases were undertaken radical nephrectomy.The tissue was then put into liquid nitrogen tank for preservation.All cancer specimens have been confirmed to be clear cell or renal carcinoma(CCRCC)by pathology.In order to investigate the differences of RUNX3 between carcinoma tissue and para-neoplastic tissue,all the samples were detected Western blot and real-time fluorescent quantitative PCR experiments and immunohistochemistry.Then the relationship of protein and mRNA level of RUNX3 were analyzed by Spearman correlation analysis and the expression of RUNX3 combined with clinical pathological data was analyzed.Result1.According to the results of Western blot,the expression RUNX3 is absolutely higher in kidney carcinoma tissue than in the tissue adjacent to carcinoma tissue.2.The result of IHC indicates that as a transcription factor,RUNX3 shows a higher expression in nucleus in carcinoma tissue,while the expression in nomal tissue is relatively low.3.RT-PCR is positively in accordance with the results of Western blot.ConclusionThe expression RUNX3 is absolutely higher in kidney carcinoma tissue than in the tissue adjacent to carcinoma tissue.Although as a tumor suppressor genes,Runx3 gene may has some potential function in the risk of renal clear cell carcinoma,but its carcinogenic mechanism is still not clear,needs to be further explored.