Screening Of Genes Related To Colon Cancer By RNA-Seq

Objective:To systematically screen key genes and the gene signal transduction pathways related to generation and development of human colon cancer using high-throughput transcriptome sequencing technologies and bioinformatic analysis, provide a theoretical basis and genomic strategy for molecular mechanism, early diagnosis, targeting therapy and prognosis prediction for human colon cancer, and build the solid foundation for the practical application of high-throughput transcriptome sequencing technologies in the field of biology and medicine through standard operating technological procedures and biological information analysis.Methods:1) Total RNA of a frozen human colon cancer tissue and the paired normal colonic tissue were extracted and the qualities of two RNA samples were detected. The integrity and purification of the total RNA isolated were tested using formaldehyde-agarose gel electrophoresis and UV spectrophotometer before two cDNA libraries were constructed.2) The Illumina Nextseq 500 sequencing platform was ued for high-throughput transcriptome sequencing. Then, the original data was sorted, filtered and quality evaluated.3) Bioinformatic analysis were used to analyze the transcriptome sequencing results (including reference genome annotation, eggNOG classification analysis, GO/KEGG enrichment analysis of different genes and cluster analysis) to identify key genes associated with generation and development of colon cancer.Results:1) In this study,1020 differentially expressed genes were identified through high-throughput transcriptome sequencing technology, of which 634 genes were up-regulated and 386 genes were down-regulated.2) Most of the differentially expressed genes were involved in signal transduction mechanism and 216 signaling pathways, including metabolics and signaling pathways in cancer. There are 23 difference genes (Wnt?Survivin?Axin? LEF-1?TCF-1?AC?ECM?PKC?PPFP?TRAFs?Glutl?TCF-7, etc.) closely related with tumor genesis and development, which associated with molecular function, cell component, cell biological process. PI3K-Akt signaling pathway, MAPK signaling pathway, p53 signaling pathway, Wnt signaling pathway, TGF? signaling pathway, cAMP signaling pathway and PPAR signaling pathway were the main signaling pathways found to correlate with the tumorigenesis of cancer.Conclusions:There are five differential genes (AC, PPFP, TCF-1, TRAFS and PKC) were found in this study to be correlated with the tumorigenesis of cancer, which have not been reported to involve in colon cancer in previous literature, suggesting they may be new biomarkers for early diagnosis and targeting therapy of colon cancer.