| Severe Fever with thrombocytopenia syndrome(SFTS)is an infectious disease discovered in recent years,and New Bunyavirus is the pathogen of the disease.Henan province is the first area to find SFTS in our country and even the world.It is also the area with the largest number of reported cases.At present,there has been no report on the antibody level and recessive infection status of New Bunyavirus in general population in Henan SFTS epidemic areas.Xinyang,as the city with the largest number of SFTS incidence in our province,is the ideal research site of SFTS.Objective:To clarify the natural infection rate and population immunization status of New Bunyavirus in the population and provide the basis for the prevention and control of SFTS in our province through investigating the antibody level and recessive infection status of New Bunyavirus inHenan SFTS epidemic areas.Methods:In this study,stratified random sampling methodwas used,and finally 7 natural villages were selected as the investigation site in Pingqiao District and Xinxian County,respectively.Serum samples were collected from the survey sites from April to May in 2016.Enzyme-linked immunosorbent assay(ELISA)was used to detect New Bunyavirus specific IgG and IgM antibodies on specimens and the results were classified according to the collected specimen information.Using statistical methods,the distribution of New Bunyavirus specific antibodies was analyzed in different genders,different age groups and different regions,.For the serum samples positive for IgM antibody,the real-time PCRwas used to detect the virus RNA,andthe cell culture technique to isolate and then identify the virus.A month after thespecimen collection,all persons positive for IgM antibody were followed up one by one to confirm whether he or shewas recessive infection.Results:A total of 1463 persons were selected from the cross-sectional survey in Xinyang City: 695 persons in Pingqiao District and 768 persons in Xinxian County;male 409 and female 1054;the age ranging from 2 to 95 years old,with a median age of 60 years old.Of 1463 people,there are 153 cases positive for New Bunyavirus IgG antibody.The total IgG positive rate was 10.46%(153/1463): Pingqiao district and Xinxian County were 7.19%(50/695)and 13.41%(103/768),respectively;the difference was statistically significant between the two areas(P<0.05);there was no significant difference in the positive rates of IgG antibody in different genders and different age groups(P>0.05).There are 12 cases positive for New Bunyavirus IgM antibody.The total IgM positive rate was 0.82%(12/1463): Pingqiao district and Xinxian County were 0.43%(3/695)and 1.17%(9/768),respectively;there was no statistically significant difference between the two areas(P>0.05);the difference of the positive rates of IgM antibody in different genders and different age groups has also no statistical significance(P>0.05).12 samples of IgM antibody positive serum samples,there were 6 samples of New Bunyavirus RNA positive samples;The virus was isolated from 5 serum samples by cell culture.The virus isolation rate was 41.67%(5/12),including 1 in Pingqiao District and 4 in Xinxian County.A month later from thetime of samples collection,all people with New Bunyavirus IgM antibody did not fail ill,in other words,they were all recessive infection.Conclusion:The level of New Bunyavirus specific IgG antibody is relatively higher and the virus epidemic intensity stronger in Xinyang City.There is latent infection orvirus carriersin the New Bunyavirus infection spectrum.It is suggested that the potential New Bunyavirus infection risk is high in the crowd. |
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