Salidroside On UVB Radiation Damage To HaCat Cells Proliferation Activity And Endogenous Apoptosis

Objective:To investigate the effects of salidroside on proliferation and endogenous apoptotic pathway of HaCat cells exposed to ultraviolet radiation（UVB）radiation,and provide the experimental basis for the clinical application and antiradiation mechanism of salidroside.Methods:HaCat cells were divided into 6 groups,Group 1 normal control group（groupⅠ）:common medium culture,no UV irradiation;Group 2 salidroside control group:adding 40μg/ml salidroside（groupⅡ）,culture medium;Group 3 UVB radiation group,normal culture medium,received 200mJ/cm² dose of ultraviolet radiation（Ⅲgroup）;Group 4,5 and 6 were treated with 10μg/ml,40μg/ml,80μg/ml（Ⅳ,Ⅴ,Ⅵ）salidroside and 200mJ/cm2 UV Irradiation.After incubation for 24 hours,the viability was measured by MTT assay,Flow cytometry was used to observe the cell cycle;The expression of Caspase-3 and Caspase-9 was detected by western blot.Results:MTT results showed that UVB irradiation could significantly inhibit the viability of HaCat cells,and Rhodiola had a protective effect and was positively correlated with drug concentration.UVB irradiation group（groupⅢ）lowest.The cell cycle of HaCat cells showed a rising trend with the increase of salidroside concentration in flow cytometry,The peak value of S（P<0.05）was higher than that of Salidroside alone（Group II）,The number of cells in groupⅢ（Ⅲ）was the highest at G0/G1 phase,and decreased in groupⅣ,ⅤandⅥ,Group high（Ⅵ）,medium（Ⅴ）and low（Ⅳ）concentration of salidroside in the experimental group showed a gradual increase trend in the G2group.The results of western blot showed that the expression of Caspase-3/9 protein was the same,and the expression of Caspase-3/9 was the highest,andⅣ,ⅤandⅥwere gradually decreased.Conclusion:Salidroside has a certain antiradiation and protective effect on HaCat cells,and its mechanism may be achieved by down-regulating the expression of endogenous apoptotic factor Caspase-3/9.