The Study Of MiR-98 On The Role Of The Repair Of The Exercise-Induced Skeletal Muscle Damage

Objective:With the popularity of lifestyle changes and public health of the masses of the Chinese people,exercise-induced muscle damage has not only in sports,it has become frequently asked questions general fitness.Damage repair after a series of problems,such as scarring,fibrosis,pain;Research found that miR-98 regulates the differentiation of skeletal muscle satellite cells,another target to become skeletal muscle damage and repair,for the treatment of exercise-induced muscle damage provided new basic theory and experimental data.Methods:Seventy-two two months old SD rats were divided into 9 groups,each group of 8.into nine group,eight per group.Group as follows:control group(C);Oh after exercise group;6h after exercise group;12h after group;24h after exercise group;48h after exercise group;72h after exercise group;1week after exercise group;2 week after group.Created by downhill race experiment model of exercise-induced skeletal muscle damage,prior to the literature and the research group experimental programme.Downhill running anesthesia directly drawn from the control group did not,downhill running groups in the movement after 0 hours,6 hours,12 hours,24 hours,48 hours,72 hours,1 week,2 weeks of narcotic materials.All intraperitoneal injection in rats using 10%of chloral hydrate anaesthesia through the inferior vena cava blood centrifugal saved after taking the serum at-80 c freezer get blood soon after taking the rectus femoris,taking muscle samples into the liquid nitrogen cooled quickly frozen isopentane 10s,placed on dry ice to chilled isopentane full flashing in ultra-low temperature freezer storage.Taking tissue samples will be conducted the following experiment:1)Take frozen HE dyed the rectus femoris(crosscutting)morphology;2)Colorimetric method and ELISA kits to detect changes in serum CK and CK-MM respectively;3)Using RT-PCR to detect miR-98,E2F5,ID1;4)E2F5,ID1 expression using Western-Blot experiment for testing.Results:1.HE staining results:Transverse Rectus Femoris earnestly in the control group showed:muscle fibers into polygons,uniform,tight rules,muscle cell nuclei evenly distributed around the cell;Histological results after exercise compared with the control group showed that muscle fibers scattered between cells become larger,irregular in shape,in the swollen State.After exercise 1W 2W and after exercise group section shows the distribution of muscle fibers to normal state,muscle fibers arranged in close proximity.2.serum CK and CK-MM:And c group compared,movement Hou Oh group serum CK of content obviously increased,has significantly differences(P<0.05);movement Hou 6h group serum CK content almost and control group flat;movement Hou 12h,and 24h,and 48h group serum CK content than control group slightly increased,no significantly differences;movement Hou 72h,and 1W,and 2W group serum CK content reduced,but no significantly differences.Movement Hou big rat serum CK-MM ELISA results displayed:and c group compared,movement Hou Oh,and 6h,and 12h group serum in the CK-MM of content increased,but differences no significantly differences;movement Hou 24h serum in the CK-MM of content significantly increased,has significantly differences;movement Hou 48h and 72h serum in the content rose,but no significantly differences;in movement Hou 1W and 2W Hou,serum CK-MM of content has basic recovery to control group level.3.sports miR-98,E2F5 and ID1 in the rectus femoris after changes in mRNA levels:And c group compared,movement Hou Oh and 6h group miR-98 mRNA of content slightly increased,differences not has significantly ;movement Hou 12h group miR-98 mRNA content declined,no significantly differences;movement Hou 24h,and 48h and 72h group miR-98 mRNA content obviously declined,has significantly differences(P<0.05),movement Hou 48h group of content minimum,movement Hou 72 hours group content began increased;movement Hou 1W group miR-98 mRNA Slightly lower than in Group c,no significant difference 2W miR-98 mRNA levels after exercise than the control group,but the difference was not significant.And c group compared,movement Hou Oh group E2F5 mRNA content increased,differences not has significantly ;movement Hou 6h,and 12h and 24h group E2F5 mRNA content obviously increased,has significantly differences(P<0.05);movement Hou 48h and 72h group E2F5 mRNA content rose,but not has significantly differences;movement Hou 1W and 2W group E2F5 mRNA content slightly reduced,almost and control group flat.And c group ID1 mRNA content compared,movement Hou Oh and 6h group ID1 mRNA content increased,differences not has statistics meaning;movement Hou 12h group ID1 mRNA content reduced,differences not has statistics meaning;movement Hou 24h,and 48h and 72h group ID1 mRNA content obviously declined,has significantly differences(P<0.05),movement Hou 48h group ID1 mRNA content minimum;movement Hou 1W and 2W group ID1 mRNA content reduced,But the difference was not statistically significant.4.after exercise in each set of Rectus Femoris E2F5,ID1 protein content changes:Compared with the c group,Oh E2F5 protein content increased after exercise,no significant difference;after exercise,6h,12h,24h and 48h E2F5 protein content increased significantly,with significant differences(P<0.05),12h Group achieved the highest after exercise;72h,1W and 2W E2F5 protein after exercise level increased slightly,do not have significant differences.And c group compared,movement Hou Oh,and 6h and 12h group unit straight muscle in the ID1 content declined,but differences not has significantly ;movement Hou 24h and 48h group unit straight muscle in the ID1 content obviously declined,has significantly differences(P<0.05);movement Hou 72h,and 1W and 2W group unit straight muscle in the ID1 content are reduced,no significantly differences,but unit straight muscle in the ID1 content began rose.Conclusion:1.a centrifugal movement for a long time can successfully establishing experimental model of exercise-induced muscle damage;2.miR-98 was inhibited in the exercise-induced muscle damage and promotes repair of skeletal muscle injury.