Research Of The Methylation Status And Clinical Significance Of The Promoter Of EYA4 Gene In Bladder Cancer

Objective : To explore the methylation status of the CpG island of Eyes Absent 4(EYA4)promoter and its significant of Clinical pathological characteristics in bladder cancer.Methods : The promoter methylation status of EYA4 gene was examined by methylation specific PCR(MSP)in 5 bladder cancer cell lines and 75 cases of primary bladder transitional cell carcinoma.1bladder immortalized epithelial cell line and 75 cases of corresponding adjacent tissues were selected as cotrols.Meanwhile,clinical pathological data of patients were investigated by statistical analysis.Results: The promoter of EYA4 were detected in 4 cases of 5 bladder cancer cell lines where methylation rate was 80%.While the methylation status is negtive in nomarl bladder immortalized epithelial cell line.The methylation rate of EYA4 gene is70.7% in bladder cancer tissues(53/75)which is significantly more frequent thanadjacent tissues(24%,18/75),and the difference was significant(c2=32.760,P<0.01).The methylation degree of the CpG island of EYA4 promoter was correlated with the clinicopathological features such as tumor diameter,tumor single and mutiple,tumor grade and stage(P<0.05).However,noassociation was detected between EYA4 methylation and age and gender(P>0.05).Conclusions:EYA4 gene CpG island of promoter region in bladder cancer cell lines and bladder cancer tissues showed a high degree of methylation,suggesting that it may be related to the occurrence and development of bladder cancer,will help the early diagnosis of bladder cancer.The methylation rate of CpG island region in EYA4 gene promoter region was closely related to the tumor diameter,tumor single and mutiple,tumor grade and stage,and had no correlation with age and sex.