The Effect Of Knockdown A20 Expression On The Proliferation,Apoptosis And Migration Of MCF-7 Cells

Purpose: Breast cancer is one of the most common malignant tumors in the world,the incidence rate is the first in women 's malignant tumors.Early detection and treatment is an effective way to reduce mortality and improve cure rate.For most patients with advanced breast cancer,it is very important to improve their cure rate and improve their quality of life.Methods:In this study,synthesized siRNA targeted to A20 gene or negative control si RNA were transfected into MCF-7 cells by using lipofectamine 2000.CCK8 assay,Annexin V and 7-AAD double staining cytometry,Transwell assay were performed to investigate the effect of knocked down A20 mRNA expression the proliferation,apoptosis,migration of MCF-7 cells,respectively.Results: A20 is produced by human umbilical vein endothelial cells stimulated by TNF(tumor necrosis factor),LPS(endotoxin)or IL-1(IL-1).Since the discovery of A20,more and more studies have confirmed that A20 can regulate the immune response and inflammatory response by modifying the protein of target cells,not only can regulate cell apoptosis and necrosis,but also as a tumor suppressor gene in some tumors.A20 is associated with breast cancer,liver cancer and other cancers.A20 is highly expressed in breast cancer resistant cell lines,highly invasive breast cancer tissues,inflammatory breast cancer cells and clinical specimens.Probably as estrogen regulated gene and potential targets for the treatment of drug-fast breast cancer and inflammatory breast cancer.Conclusion: It was found that the proliferation and migration of breast cancer cell line MCF-7 were inhibited,and the apoptosis of the cells was promoted.The biological effect of the A20 gene abnormal expression in MCF-7 cell lines was verified,the research laid the foundation for the study of the signal pathway of A20 gene,it also provides a potential target for anti-tumor targeted therapy of breast cancer.