Studies On Preparation And Properties Of Two Novel Porous Bone Tissue Engineering Scaffolds

Background & Objective: For a long time, the repair of segmental bone defects has not been effectively solved, and it has become an urgent problem to be solved by orthopedic surgeons. The rapid development of bone tissue engineering for clinical bone defects provides a new way of thinking. Scaffold for bone tissue engineering is the most basic one of the components, how to build an ideal scaffold has been a hot research field of bone tissue engineering. This study was based on different materials,the preparation of two new porous bone tissue engineering scaffold: 3D printing porous titanium scaffold (3D-STPT) and a porous ceramic scaffold reinforced BCP(RBCP); two new morphology of porous bone tissue engineering scaffolds and aspect of the microstructure, pore size and porosity, mechanical properties and compatibility with biological tissues and other biological detection; preliminary assessment of bone defects two new porous bone tissue engineering scaffold repair capacity by constructing a critical radial rabbit forearm bone defect model.Materials and methods: 1. 3D printing porous titanium support (3D-STPT) and reinforced BCP porous ceramic support (RBCP) were prepared by 3D printing,surface chemical treatment, electrochemical deposition and hydrogen peroxide foaming method respectively. appearance and microstructure, pore size and porosity,to evaluate the biomechanical properties. 2. MC3T3-E1 cells and in vitro co-culture of two new porous bone tissue engineering scaffold for assessment of biological tissue compatibility, biocompatibility preliminary evaluation of two novel porous bone tissue engineering scaffolds. The bone defect repair ability of two new types of porous bone tissue engineering scaffolds was evaluated by constructing the critical bone defect model of rabbit radius.Results: 1. SEM scans showed: two novel porous bone tissue engineering scaffold possess a porous structure, associated opening holes with a diameter and porosity:porous titanium scaffold 3D printing (3D-STPT) pore diameter in the range 400pm-500?m, porosity was (66.0±0.7)%; BCP reinforced porous ceramic support(RBCP) pore diameter in the range 100?m-500?m, a porosity (74.6±3.0)%,substantially to reach the bone tissue engineering scaffold porosity and pore size of the basic requirements (pore size>100?m, a porosity>60%); X-ray diffraction results showed that: two surfactants component and component design theory novel porous bone tissue engineering scaffold consistent; biomechanical test results show that: a porous titanium bracket 3D printing (3D-STPT) compressive strength of 83.14±0.96MPa, reinforced BCP porous ceramic support (RBCP) a compressive strength of 7.68±0.23MPa, two novel porous bone tissue engineering scaffold for bone tissue engineering scaffold meet the biomechanical performance criteria. 2. CCK-8 results showed that two kinds of new porous tissue engineering scaffolds could promote the proliferation of MC3T3-E1 cells in vitro and had better biocompatibility. 3. Rabbit radial bone repair experiments show that: two new porous bone tissue scaffold in the animal body critical bone defect has a good role in repairing bone defects.Conclusion: 1. (3D-STPT) and reinforced BCP porous ceramic stent (RBCP) were successfully prepared based on two different materials and 3D printing techniques.The pore size, Porosity and biomechanical properties were in accordance with the requirements of bone tissue engineering scaffolds. 2. Two new porous tissue engineering scaffolds had good biocompatibility; 3. Two new porous bone tissue scaffolds could repair rabbit radial Sexual bone defects, with potential clinical application value.