Effect Of Immunosuppressor MT01 On The Expression Of TLR9?TRAF6 And IL-6 After Orthodontic Tooth Movement

Orthodontic tooth movement is the process that bone absorb in the pressure lateral and form in the tension lateral during the bone remodeling,many immune components such as inflammatory factors,growth factors are involved in orthodontic tooth movement process.Therefore,most of the tooth movement is considered to belong to the inflammatory reaction process.MT01 is an immunosuppressive ODN.Previous studies have shown that MT01 can inhibit the activation of TLR9 caused by the body's inflammatory response to prevent tissue cells from damage,and can promote osteoblast differentiation of alveolar bone in the process of tooth movement,and inhibit osteoclast activation.The question of whether TLR9 is involved in the process of initiating orthodontic tooth alveolar bone inflammation,of whether MT01 can inhibit the TLR9 signaling pathway and further inhibit the inflammatory response during tooth movement have to be investigate.In the background of this study,it is hoped that the following experimental study will provide a tentative experimental basis and possible mechanisms for the further study of MT01 on the regulation of the periodontal tissue immune response in orthodontic tooth movement.OBJECTIVE:To investigate the expression of TLR9,TRAF6 and its downstream inflammatory factor IL-6 in periodontal tissues during the period of experimental tooth movement,and to explore the effect of MT01 on TLR9,TRAF6 and IL-6 expression level,so as to evaluate the effect of MT01 on alveolar bone remodeling and periodontal physiological response after orthodontic tooth movement in rats.Methods:Seventy-two male Wistar rats were randomly divided into three groups: non-drug intervention group(n = 36)and drug intervention group(n = 36),0.49 N force loaded on the maxillary first molar for 3,7,14,21 days to death.The relative expression ofTLR9,TRAF6 and IL-6 m RNA was detected by real-time quantitative PCR.The other three groups were treated with cardiac perfusion The paraffin sections were made after maxillary first molar and its surrounding periodontal tissues.The morphological changes of periodontal tissues were observed by HE staining.Results:?Without MT01 intervening,the expression levels of TLR9,TRAF6 and IL-6m RNA in the tension lateral were significantly higher than those in the control group(P <0.01).TLR9,TRAF6 and IL-6 m RNA in the compression lateral were significantly higher than those in the control group on 7,14,21days(P<0.01).The expression of IL-6m RNA was significantly higher than that of the control group(P<0.01),and the relative expression of each factor in the pressure lateral and the tension lateral both reached the peak at day 7;?When intervened by MT01,the relative expression of TLR9,TRAF6 and IL-6 in the tension lateral was not significantly different from that of the control group on day3(P<0.05)and the expression of TLR9 and IL-6 was significantly lower than that of the control group on 14,21 days(P<0.01),but the expression of TRAF6 was not significantly different from that of the control group.The relative expression of TLR9 and IL-6 in the compression lateral was significantly decreased at 3 days.The expression of TLR9,TRAF6 and IL-6 Significantly lower than the control group on21 day(P <0.01);?The results of HE staining showed that the degree of alveolar bone resorption in the first molar was lighter than that in the control group,and the osteogenesis in tension lateral was active compared with the control group.Conclusion:The expression of TLR9,TRAF6 and its downstream inflammatory factor IL-6in periodontal tissues of experimental tooth movement is increased.MT01 can inhibit the expression of TLR9 and downstream related factors in periodontal tissues during orthodontic tooth movement.