The Study Of Molecular Mechanism Of The Regulation Of Innate Immunity By Post-translational Modification Neddylation

Objective:To investigate the mechanism of Neddylation in regulating the innate immunity response.Methods:1.Luciferase experiment tested the function of MLN4924 regulating IFN production activated by Se V.2.The overexpression of Neddylation E2 proteins UBE2 M mutant and UBE2 F mutantaffected IFN production signaling pathway induced by Se V.3.Luciferase experiment tested the role of MLN4924 on IFN production activated by RNA sensing.4.Luciferase experiment detected the role of MLN4924 on regulating IFN production activated by RLR signaling pathway effectors.5.To examine the effect of MLN4924 controlling endogenous IFN production signaling pathway by RT-PCR experiment.Results:1.MLN4924 inhibited IFN production induced by Se V.2.The Neddylation E2 protein UBE2 M and UBE2 F regulated IFN production signaling pathway.3.MLN4924 inhibited the activation of RNA-induced IFN production signaling pathway.4.MLN4924 interdicted the IFN production signaling pathway induced by the effectors of RLR signaling pathway.5.MLN4924 suppressed the endogenous IFN production signaling pathway.Conclusions:1.Neddylation inhibited the activition of innate immune response.2.Neddylation regulated the RLR signaling pathway.3.UBE2 M was the crucial effectors of Neddylation regulating the innate immune response.